Isolation and Identification of Severe Fever with Thrombocytopenia Syndrome Virus from Farmed Mink in Shandong, China

文献类型： 外文期刊

第一作者： Meng, Xiangshu

作者： Meng, Xiangshu;Sun, Jian;Yao, Mengfan;Xu, Han;Liu, Chao;Chen, Han;Guo, Jie;Nie, Xiaoxuan;He, Longbin;Wang, Jianke;Sun, Jian;Sun, Yue;Zhao, Zongzheng;Li, Nan;Wang, Zekun

作者机构：

期刊名称：TRANSBOUNDARY AND EMERGING DISEASES （ 影响因子：4.3； 五年影响因子：4.3 ）

ISSN： 1865-1674

年卷期： 2024 年 2024 卷

页码：

收录情况： SCI

摘要： Severe fever with thrombocytopenia syndrome (SFTS) virus, recently named Bandavirus dabieense, belongs to the genus Bandavirus of family Phenuiviridae, and it causes SFTS in humans with clinical symptoms including fever, thrombocytopenia, gastrointestinal symptoms, and leukocytopenia. However, there are few reports on the pathogenesis of SFTSV in animals. This study first isolated the SFTSV strain SD22-2 from sick-farmed mink. Viral metagenomics was used to detect SFTSV nucleotide in the clinical specimens obtained from symptomatic minks. Then, we isolated the virus using Vero and DH82 cells, and Real-Time Quantitative PCR (RT-qPCR), indirect immunofluorescence assay, transmission electron microscopy, and Western blotting identified it. Meanwhile, phylogenetic analysis based on partial L, M, and S segment sequences indicated that the mink-origin SFTSV strain SD22-2 belonged to genotype D and was genetically close to the HB2016-003 strain isolated from humans. Taken together, we isolated and identified an SFTSV from farmed mink that may be the reservoir hosts of SFTSV. We should pay more attention to farmed minks and biosecurity practices, and active surveillance at fur farms must be reviewed and enhanced.

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