Visual detection of fowl adenovirus serotype 4 via a portable CRISPR/Cas13a-based lateral flow assay

文献类型： 外文期刊

第一作者： Yin, Dongdong

作者： Yin, Dongdong;Yin, Lei;Wang, Jieru;Dai, Yin;Shen, Xuehuai;Zhao, Ruihong;Pan, Xiaocheng;Yin, Dongdong;Qi, Kezong

作者机构：

关键词： Fowl adenovirus serotype 4; RAA; CRISPR/Cas13a; SHERLOCK; collateral cleavage; lateral flow detection

期刊名称：AVIAN PATHOLOGY （ 影响因子：2.8； 五年影响因子：3.2 ）

ISSN： 0307-9457

年卷期： 2023 年 52 卷 6 期

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收录情况： SCI

摘要： The widespread occurrence of fowl adenovirus serotype 4 (FAdV-4)-induced hepatitis-hydropericardium syndrome (HHS) has led to significant economic losses for the poultry industry. A sensitive, accurate, and practical FAdV-4 diagnostic approach is urgently required to limit the incidence of the disease. In the present study, a practical method for detecting FAdV-4 was developed using the CRISPR/Cas13a system and recombinase-aided amplification. The approach was based on 37 degrees C isothermal detection with visible results being achieved. The detection limit of the target gene with this approach was only 10(1) copies/mu l, making it very sensitive and specific. Clinical samples fared well when tested with the Cas13a detection method. For identifying FAdV-4, this novel detection approach was found to be sensitive, specific, and effective.

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