Fast and Ultrasensitive Visual Detection of Exosomes in Body Fluids for Point-of-Care Disease Diagnosis

文献类型： 外文期刊

第一作者： Chen, Shan

作者： Chen, Shan;Jiang, Tao;Chen, Junyan;Yang, Shuangli;Wang, Pengcheng;Sun, Junjie;Yin, Congcong;Huang, Zongxiong;Fang, Yimin;Chen, Shan;Lin, Hao;Gan, Xinqiang;Wang, Yali;Xu, Bin

作者机构：

期刊名称：ANALYTICAL CHEMISTRY （ 影响因子：6.986； 五年影响因子：6.755 ）

ISSN： 0003-2700

年卷期： 2021 年 93 卷 29 期

页码：

收录情况： SCI

摘要： Fast detection of low-concentration exosomes in body fluids is of great significance in understanding the pathogenesis and disease diagnosis but is quite a challenging work due to the complex matrix, tedious pretreatment, and relatively poor sensitivity without the aid of instruments. In this work, by simply using a filter membrane to enrich the exosomes at low concentrations and the use of CuS nanoparticles as labels, we were able to detect exosomes at concentrations as low as 2 x 10(3) particles/mu L in a complex matrix by the naked eye. Due to its high sensitivity, specificity, and simplicity, it can be used for the diagnosis of direct prostate cancer via a 5 mL urine sample within 2 h without the use of any instrument. This method can also be applicable for the detection of other biological nanoparticles, such as viruses, at low concentrations in a complex matrix, offering a promising candidate for point-of-care disease diagnosis with low cost.

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