Disruption of OsPHD1, Encoding a UDP-Glucose Epimerase, Causes JA Accumulation and Enhanced Bacterial Blight Resistance in Rice

文献类型： 外文期刊

第一作者： Gao, Yu

作者： Gao, Yu;Xiang, Xiaojiao;Zhang, Yingxin;Cao, Yongrun;Wang, Beifang;Zhang, Yue;Wang, Chen;Jiang, Min;Duan, Wenjing;Chen, Daibo;Zhan, Xiaodeng;Cheng, Shihua;Liu, Qunen;Cao, Liyong;Gao, Yu;Xiang, Xiaojiao;Zhang, Yingxin;Cao, Yongrun;Wang, Beifang;Zhang, Yue;Wang, Chen;Jiang, Min;Duan, Wenjing;Chen, Daibo;Zhan, Xiaodeng;Cheng, Shihua;Liu, Qunen;Cao, Liyong;Cao, Liyong

作者机构：

关键词： rice (Oryza sativa L; ); lesion mimic mutant; programmed cell death; defense response

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：6.208； 五年影响因子：6.628 ）

ISSN：

年卷期： 2022 年 23 卷 2 期

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收录情况： SCI

摘要： Lesion mimic mutants (LMMs) have been widely used in experiments in recent years for studying plant physiological mechanisms underlying programmed cell death (PCD) and defense responses. Here, we identified a lesion mimic mutant, lm212-1, which cloned the causal gene by a map-based cloning strategy, and verified this by complementation. The causal gene, OsPHD1, encodes a UDP-glucose epimerase (UGE), and the OsPHD1 was located in the chloroplast. OsPHD1 was constitutively expressed in all organs, with higher expression in leaves and other green tissues. lm212-1 exhibited decreased chlorophyll content, and the chloroplast structure was destroyed. Histochemistry results indicated that H2O2 is highly accumulated and cell death is occurred around the lesions in lm212-1. Compared to the wild type, expression levels of defense-related genes were up-regulated, and resistance to bacterial pathogens Xanthomonas oryzae pv. oryzae (Xoo) was enhanced, indicating that the defense response was activated in lm212-1, ROS production was induced by flg22, and chitin treatment also showed the same result. Jasmonic acid (JA) and methyl jasmonate (MeJA) increased, and the JA signaling pathways appeared to be disordered in lm212-1. Additionally, the overexpression lines showed the same phenotype as the wild type. Overall, our findings demonstrate that OsPHD1 is involved in the regulation of PCD and defense response in rice.

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