Rice-produced classical swine fever virus glycoprotein E2 with herringbone-dimer design to enhance immune responses

文献类型： 外文期刊

第一作者： Xu, Qianru

作者： Xu, Qianru;Xu, Qianru;Ma, Fanshu;Zhang, Longxian;Hu, Hui;Li, Xueyang;Zhang, Shenli;Du, Yongkun;Zhang, Erqin;Zhang, Gaiping;Xu, Qianru;Li, Qingmei;Liu, Yunchao;Li, Rui;Wei, Qiang;Wang, Yanan;Yang, Jifei;Chai, Shujun;Wang, Li;Zhang, Gaiping;Ma, Fanshu;Yang, Daichang;Yang, Daichang;Ou, Jiquan;Zhan, Quan;Yan, Liming;Zhang, Longxian;Zhang, Erqin;Zhang, Gaiping;Zhang, Gaiping

作者机构：

关键词： transgenic rice; rational vaccine design; herringbone-dimer; Pestivirus; E2 protein

期刊名称：PLANT BIOTECHNOLOGY JOURNAL （ 影响因子：13.8； 五年影响因子：13.2 ）

ISSN： 1467-7644

年卷期： 2023 年

页码：

收录情况： SCI

摘要： Pestiviruses, including classical swine fever virus, remain a concern for global animal health and are responsible for major economic losses of livestock worldwide. Despite high levels of vaccination, currently available commercial vaccines are limited by safety concerns, moderate efficacy, and required high doses. The development of new vaccines is therefore essential. Vaccine efforts should focus on optimizing antigen presentation to enhance immune responses. Here, we describe a simple herringbone-dimer strategy for efficient vaccine design, using the classical swine fever virus E2 expressed in a rice endosperm as an example. The expression of rE2 protein was identified, with the rE2 antigen accumulating to 480 mg/kg. Immunological assays in mice, rabbits, and pigs showed high antigenicity of rE2. Two immunizations with 284 ng of the rE2 vaccine or one shot with 5.12 lg provided effective protection in pigs without interference from pre-existing antibodies. Crystal structure and small-angle X-ray scattering results confirmed the stable herringbone dimeric conformation, which had two fully exposed duplex receptor binding domains. Our results demonstrated that rice endosperm is a promising platform for precise vaccine design, and this strategy can be universally applied to other Flaviviridae virus vaccines.

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