Epigoitrin Exerts in vitro anti-MRV Effect via Activation of Interferon-Stimulated Genes

文献类型： 外文期刊

第一作者： Tao, Ziqian

作者： Tao, Ziqian;Tao, Ziqian;Ma, Yanping;Hao, Le;Jia, Mengyan;Liu, Zhenxing;Ma, Yanping;Hao, Le;Jia, Mengyan;Liu, Zhenxing;Huang, Wen;Tao, Ziqian;Ma, Yanping;Hao, Le;Jia, Mengyan;Huang, Wen;Liu, Zhenxing

作者机构：

关键词： epigoitrin; mandarin fish ranavirus; interferon-stimulated genes

期刊名称：FISH PATHOLOGY （ 影响因子：0.2； 五年影响因子：0.4 ）

ISSN： 0388-788X

年卷期： 2024 年 59 卷 2 期

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收录情况： SCI

摘要： The mandarin fish ranavirus (MRV), classified within the genus Ranavirus of the Iridoviridae family, is a lethal pathogen to Mandarin fish ( Siniperca chuatsi). ). Currently, no approved treatment exists, underscoring the urgent need for therapeutic strategies. In this study, we aimed to investigate the antiviral activity of epigoitrin against MRV. An in vitro antiviral activity assay revealed that the CC50 of epigoitrin is 43.16 mu M , as determined by the CCK-8 assay. Epigoitrin effectively reduced the number of MRV copies from 8.63 x 10(8) copies/mL to 1.82 x 10(8) copies/mL, exhibiting dose-dependent inhibition of MRV replication. The effective concentration (EC50) of epigoitrin was further determined to be 13.72 mu M . Moreover, epigoitrin diminished the MRV-induced cytopathic effect as observed under a light microscope and suppressed the expression of major caspid protein of MRV (MRV-MCP), as visualized by immunofluorescence assay. It was revealed that epigoitrin primarily exerts its antiviral effects during the stage of viral replication. Further investigation into the immune response revealed that epigoitrin significantly upregulated the gene expression of Mx1 (5-fold), IFITM (2.5-fold), viperin (10- fold), ISG15 (15-fold), and IRF5 (2-fold). This suggests that epigoitrin indirectly unleashes its antiviral function by activating the host's interferon (IFN) immune response. In conclusion, our findings indicate that epigoitrin holds great potential as a therapeutic agent against MRV infection by modulating the IFN pathway.

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