Structural insights into the oligomeric effects on catalytic activity of a decameric feruloyl esterase and its application in ferulic acid production

文献类型： 外文期刊

第一作者： Du, Guoming

作者： Du, Guoming;Wang, Yulu;Yu, Haiyan;Liu, Shujun;Ma, Xiaochen;Cao, Hao;Wei, Xue;Wen, Boting;Li, Zhen;Xin, Fengjiao;Zhang, Yuebin;Fan, Shilong;Zhou, Huan;Wang, Yulu;Liu, Shujun;Wen, Boting;Li, Zhen;Xin, Fengjiao

作者机构：

关键词： Feruloyl esterase; Cold-adapted; Oligomerization

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.2； 五年影响因子：7.8 ）

ISSN： 0141-8130

年卷期： 2023 年 253 卷

页码：

收录情况： SCI

摘要： Oligomeric feruloyl esterase (FAE) has great application prospect in industry due to its potentially high stability and fine-tuned activity. However, the relationship between catalytic capability and oligomeric structure remains undetermined. Here we identified and characterized a novel, cold-adapted FAE (BtFae) derived from Bacteroides thetaiotaomicron. Structural studies unraveled that BtFae adopts a barrel-like decameric architecture unique in esterase families. By disrupting the interface, the monomeric variant exhibited significantly reduced catalytic activity and stability toward methyl ferulate, potentially due to its impact on the flexibility of the catalytic triad. Additionally, our results also showed that the monomerization of BtFae severely decreased the ferulic acid release from de-starched wheat bran and insoluble wheat arabinoxylan by 75 % and 80 %, respectively. Collectively, this study revealed novel connections between oligomerization and FAE catalytic function, which will benefit for further protein engineering of FAEs at the quaternary structure level for improved industrial applications.

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