Chimeric mutations in grapevine ENHANCED DISEASE RESISTANCE1 improve resistance to powdery mildew without growth penalty

文献类型： 外文期刊

第一作者： Yu, Xue-Na

作者： Yu, Xue-Na;Guo, Ye;Yang, Qianling;Yu, Haiyan;Lu, Meng-Jiao;Zhao, Liang;Jin, Zhuo-Shuai;Feng, Jia-Yue;Wen, Ying-Qiang;Yu, Xue-Na;Guo, Ye;Yang, Qianling;Yu, Haiyan;Lu, Meng-Jiao;Zhao, Liang;Jin, Zhuo-Shuai;Feng, Jia-Yue;Wen, Ying-Qiang;Xu, Xiang-nan

作者机构：

期刊名称：PLANT PHYSIOLOGY （ 影响因子：6.5； 五年影响因子：7.6 ）

ISSN： 0032-0889

年卷期： 2024 年 195 卷 3 期

页码：

收录情况： SCI

摘要： Grapevine (Vitis vinifera L.) incurs severe quality degradation and yield loss from powdery mildew, a major fungal disease caused by Erysiphe necator. ENHANCED DISEASE RESISTANCE1 (EDR1), a Raf-like mitogen-activated protein kinase kinase kinase, negatively regulates defense responses against powdery mildew in Arabidopsis (Arabidopsis thaliana). However, little is known about the role of the putatively orthologous EDR1 gene in grapevine. In this study, we obtained grapevine VviEDR1-edited lines using CRISPR/Cas9. Plantlets containing homozygous and bi-allelic indels in VviEDR1 developed leaf lesions shortly after transplanting into the soil and died at the seedling stage. Transgenic plants expressing wild-type VviEDR1 and mutant Vviedr1 alleles as chimera (designated as VviEDR1-chi) developed normally and displayed enhanced resistance to powdery mildew. Interestingly, VviEDR1-chi plants maintained a spatiotemporally distinctive pattern of VviEDR1 mutagenesis: while almost no mutations were detected from terminal buds, ensuring normal function of the apical meristem, mutations occurred in young leaves and increased as leaves matured, resulting in resistance to powdery mildew. Further analysis showed that the resistance observed in VviEDR1-chi plants was associated with callose deposition, increased production of salicylic acid and ethylene, H2O2 production and accumulation, and host cell death. Surprisingly, no growth penalty was observed with VviEDR1-chi plants. Hence, this study demonstrated a role of VviEDR1 in the negative regulation of resistance to powdery mildew in grapevine and provided an avenue for engineering powdery mildew resistance in grapevine. ENHANCED DISEASE RESISTANCE1 negatively regulates powdery mildew resistance in grapevine, and chimeric edited lines improve resistance without growth penalty.

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