Novel insights into the inhibitory effect of pterostilbene targeting Aeromonas hydrophila virulence

文献类型： 外文期刊

第一作者： Dong, Jing

作者： Dong, Jing;Zhou, Shun;Liu, Yongtao;Yang, Qiuhong;Yang, Yibin;Xu, Ning;Ai, Xiaohui

作者机构：

关键词： Pterostilbene; Aerolysin; Quorum sensing; Molecular dynamics; Aeromonas hydrophila

期刊名称：AQUACULTURE （ 影响因子：3.9； 五年影响因子：4.1 ）

ISSN： 0044-8486

年卷期： 2024 年 590 卷

页码：

收录情况： SCI

摘要： The overuse of antibiotics in aquaculture brings a range of problems. Therefore, novel approaches alternating antibiotics are urgently needed. Strategies targeting bacterial virulence have become one of the routes developing drugs against bacterial infections. Here, we found that pterostilbene (PSE) could inhibit the activity of aerolysin (AerA) and disrupt quorum sensing (QS) at sub-inhibitory concentrations. Molecular docking and dynamics simulation assays were performed to predict the binding mode and binding sites of AerA-PSE complex. Then we confirmed that PSE bound to the sites of Val189 and Ile302 and prevented AerA forming heptamer with transmembrane pores. In addition, PSE could protect to A549 cells and the channel catfish (Ictalurus punctatus) against AerA mediated cell injury and mortality infected with A. hydrophila. The findings demonstrated that targeting bacterial virulence could be a promising measure identifying agents against A. hydrophila infections.

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