Efficacy and safety assessment of triclabendazole for treating Gyrodactylus infections in goldfish

文献类型： 外文期刊

第一作者： Zhou, Shun

作者： Zhou, Shun;Dong, Jing;Liu, Yongtao;Yang, Qiuhong;Zhang, Lang;Ai, Xiaohui;Chen, Shengao;Xia, Liwei

作者机构：

关键词： Triclabendazole; Anthelmintic activity; Safety; Gyrodactylids

期刊名称：AQUACULTURE （ 影响因子：4.5； 五年影响因子：4.6 ）

ISSN： 0044-8486

年卷期： 2024 年 584 卷

页码：

收录情况： SCI

摘要： Gyrodactylus spp. pose a major threat to aquaculture due to their rapid proliferation and ability to cause severe infections in farmed fish. The existing treatments currently available have notable limitations, necessitating the exploration of new anthelmintic strategies. In this trial, in vitro and in vivo anthelmintic activity of triclabendazole against Gyrodactylus kobayashii and its safety in goldfish (Carassius auratus) were evaluated. The in vitro assays demonstrated a rapid increase in the mortality of G. kobayashii with increasing concentrations of triclabendazole, with the complete death of the worms achieved within 4 and 3 h at 0.1 and 0.2 mg/L, respectively. Triclabendazole demonstrated time- and concentration-dependent anthelmintic activity in vivo, with complete elimination of worms from goldfish achieved at 0.1 mg/L after 48 h of exposure. The EC50 values after 24 and 48 h of exposure were 0.055 and 0.047 mg/L, respectively. To further elucidate the mechanism of action of triclabendazole against G. kobayashii, scanning electron microscopy and worm movement observation after exposure to triclabendazole were conducted. Exposure to triclabendazole induced reduced mobility and noticeable tegumental damages, potentially responsible for the death of worms. The 48-h LC50 value of triclabendazole against goldfish was 1.12 mg/L and the therapeutic index (LC50/EC50) was calculated to be 23.83 after 48 h of exposure. Histopathological analysis indicated minor modifications were detected in the gill and liver tissues after triclabendazole exposure. The expressions of cyp1a, hsp70, gst, and sod were markedly modified during the 6 or 48-h post-exposure period, but tended to return to baseline levels with longer exposure. Overall, triclabendazole demonstrated superior anthelmintic activity against gyrodactylids coupled with an adequate safety profile in goldfish. These results supported the further development of triclabendazole as an alternative treatment for gyrodactylid infections in aquaculture.

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