sRNA STnc150 is involved in virulence regulation of Salmonella Typhimurium by targeting fimA mRNA

文献类型： 外文期刊

第一作者： Li, Jing

作者： Li, Jing;Li, Na;Ning, Chengcheng;Guo, Yun;Ji, Chunhui;Zhu, Xiaozhen;Meng, Qingling;Shang, Yunxia;Xiao, Chencheng;Xia, Xianzhu;Qiao, Jun;Zhang, Xingxing;Cai, Xuepeng

作者机构：

关键词： STnc150; virulence regulation; Salmonella typhimurium; fimA mRNA

期刊名称：FEMS MICROBIOLOGY LETTERS （ 影响因子：2.82； 五年影响因子：3.248 ）

ISSN： 0378-1097

年卷期： 2021 年 368 卷 18 期

页码：

收录情况： SCI

摘要： Small RNAs (sRNAs) are essential virulent regulators in Salmonella typhimurium (STM). To explore the role of sRNA STnc150 in regulating STM virulence, we constructed a STnc150 deletion strain (Delta STnc150) and its complementary strain (Delta STnc150/C). Then, we compared their characteristics to their original parent strain experimentally, identified the target genes of STnc150 and determined the expression levels of target genes. The results showed that the Delta STnc150 strain exhibited delayed biofilm formation, enhanced adhesion to macrophages, significantly reduced LD50, increased liver and spleen viral loads and more vital pathological damaging ability than its parent and complementary strains. Further, bioinformatics combined with the bacterial dual plasmid reporter system confirmed that the bases 72-88 of STnc150 locating at the secondary stem-loop structure of the STnc150 are complementary with the bases 1-19 in the 5'-terminal of fimA mRNA of the type 1 fimbriae subunit. Western blot analysis showed that fimA protein level was increased in STnc150 strain compared with its parent and complementary strains. Together, this study suggested that STnc150 can down-regulate STM fimA expression at the translation level, which provided insights into the regulatory mechanisms of sRNAs in virulence of STM.

分类号：

  • 相关文献

[1]sRNA STnc150 is involved in virulence regulation of Salmonella Typhimurium by targeting fimA mRNA. Li, Jing,Li, Na,Ning, Chengcheng,Guo, Yun,Ji, Chunhui,Zhu, Xiaozhen,Meng, Qingling,Shang, Yunxia,Xiao, Chencheng,Xia, Xianzhu,Qiao, Jun,Zhang, Xingxing,Cai, Xuepeng. 2021

作者其他论文 更多>>

微信小程序