First genome-wide association and genomic prediction of ammonia-nitrogen tolerance in tiger pufferfish (Takifugu rubripes)

文献类型： 外文期刊

第一作者： Chi, Yong

作者： Chi, Yong;Hu, Yuanri;Chen, Songlin;Li, Yangzhen;Chi, Yong;Chen, Songlin;Li, Yangzhen;Chi, Yong;Li, Weidong;Li, Yangzhen;Mukiibi, Robert;Robledo, Diego;Robledo, Diego

作者机构：

关键词： Fugu; Heritability; Environmental stress; GWAS; QTL; Aquaculture

期刊名称：AQUACULTURE （ 影响因子：3.9； 五年影响因子：4.4 ）

ISSN： 0044-8486

年卷期： 2025 年 600 卷

页码：

收录情况： SCI

摘要： Ammonia-nitrogen stress is a significant threat to the growth and survival of tiger pufferfish (Takifugu rubripes), especially in high-density stocking integrated aquaculture systems. This study aimed to decipher the genetic background of ammonia-nitrogen stress tolerance via heritability estimation and identification of variants associated with this stress via genome-wide association studies (GWAS). We also assessed the potential of applying genomic selection (GS) to improve this trait in T. rubripes. A customized a low-density 20 K SNP array was used to genotype 780 fish, and low-density genotypes were imputed to a high-density genotype dataset comprising over 1.3 million SNPs. The heritability of ammonia-nitrogen tolerance was estimated as 0.20 and 0.15 and 0.21 for pedigree, low-density and high-density SNP markers panels, respectively. A suggestively significant quantitative trait locus (QTL) was detected on chromosome 8, containing 16 significant SNPs within a 70.0 kb region. Notably, two candidate genes, fgf11A and serinc5, were associated with ammonia-nitrogen stress tolerance. Use of different genomic prediction models to estimate genetic merit of selection candidates showed improvement in accuracy of breeding value estimation by at least 57 % compared to traditional pedigree-based methods. Interestingly, the prediction remained significantly high even with the lowest number of panel markers (500 markers). These findings highlight the potential of GS to enhance ammonia-nitrogen stress tolerance in T. rubripes breeding programs, thus contributing to the sustainability and productivity of the species.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序