Transgene directionally integrated Into C-genome of Brassica napus

文献类型： 外文期刊

第一作者： Li, Jun

作者： Li, Jun;Fang, Xiaoping;Wang, Zhuan;Li, Jun;Luo, Lixia;Hu, Qiong

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关键词： Bar gene;C-genome;directional transgene;resynthesis of Brassica napus

期刊名称：CHINESE SCIENCE BULLETIN （ 影响因子：1.649； 五年影响因子：1.738 ）

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收录情况： SCI

摘要： Integration of a transgene into a C-genome chromosome plays an important role in reducing ecological risk of transgenic Brassica napus. To obtain C-genome transgenic B. napus, herbicide-resistant bar gene was firstly transferred into B. oleracea var. alboglabra mediated by Agrobacterium tumefaciens strain LBA4404. Then using the transgenic B. oleracea as paternal plants and 8 non-transgenic varieties of 8. rapa as maternal plants, C-genome transgenic B. napus with bar gene was artificially resynthesized by means of ovary culture and chromosome doubling. Among 67 lines of the resynthesized B. napus, 31 were positive, and 36 were negative according to PCR test for bar gene. At least 2 plants from each line were kept for PPT spray confirmation. The result was in consistence with the PCR test. Genomic Southern blotting of three randomly chosen lines also showed that bar gene had been integrated into the genome of resynthesized B. napus lines.

分类号： N1

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