Disruption effect of Microplitis bicoloratus polydnavirus EGF-like protein, MbCRP, on actin cytoskeleton in lepidopteran insect hemocytes

文献类型： 外文期刊

第一作者： Luo, Kai-Jun

作者： Luo, Kai-Jun;Pang, Yi

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关键词： Amino Acid Sequence;Animals;Baculoviridae genetics;Base Sequence;Cell Line;Cloning;Molecular;DNA;Viral chemistry;Epidermal Growth Factor genetics;Female;Hemocytes metabolism parasitology virology;Host-Parasite Relations;Larva parasitology virology;Lepidoptera cytology parasitology virology;Microfilaments metabolism;Molecular Sequence Data;Polydnaviridae genetics ultrastructure;RNA;Messenger metabolism;Research Support;Non-U.S. Gov't;Spodoptera growth & development parasitology virology;Viral Proteins genetics metabolism;Virion ultrastructure;Wasps physiology virology

期刊名称：ACTA BIOCHIMICA ET BIOPHYSICA SINICA （ 影响因子：3.848； 五年影响因子：3.67 ）

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收录情况： SCI

摘要： Microplitis bicoloratus is a braconid endoparasitic wasp associated with the polydnavirus named Microplitis bicoloratus bracovirus (MbBV). Parasitism of Spodoptera litura larvae leads to an impaired cellular immune response and to the disappearance of the 42 kDa actin in host hemocytes. In this work, we investigated if the absence of actin in blood cells was related to MbBV infection. An MbBV gene similar to egf-like genes identified in another bracovirus was partially cloned and named Mbcrp1. The full-length gene, named Mbcrp, is transcribed throughout the course of parasitism in host hemocytes and the 30 kDa MbCRP protein was detected in hemocytes 6-7 d post-parasitization. The Mbcrp1 gene contains the cysteine-rich trypsin inhibitor-like (TIL) domain coding sequence and the expression of recombinant MbCRP1 inhibited the expression of the 42 kDa actin in Hi5 cells. The 34.1 kDa MbCRP1-green fluorescent protein fusion protein locate specifically in the cytoplasm. These results suggest that expression of MbCRP in lepidopteran insect cells is related to the disruption of the actin cytoskeleton.

分类号： Q7

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