Cryo-EM structure reveals cylindrical nucleocapsids from two polydnaviruses

文献类型： 外文期刊

第一作者： Cui, Ji-Hui

作者： Cui, Ji-Hui;Chen, Ya-Bin;Li, Ming;Cai, Qiu-Chen;Zhang, Li-Dan;Zhu, Qi-Shun;Luo, Kai-Jun;Cui, Ji-Hui;Li, Ming;Cai, Qiu-Chen;Zhang, Li-Dan;Luo, Kai-Jun;Cui, Ji-Hui;Chen, Ya-Bin;Li, Ming;Cai, Qiu-Chen;Zhang, Li-Dan;Zhu, Qi-Shun;Luo, Kai-Jun;Lu, Zi-Yun;Li, Jian-Cheng;Ji, Gang

作者机构：

期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.574； 五年影响因子：2.466 ）

ISSN： 0304-8608

年卷期： 2018 年 163 卷 12 期

页码：

收录情况： SCI

摘要： Bracovirus is one of the two polydnavirus genera. Here, we used a cryo-EM analysis to reveal the near-native morphology of two nucleocapsid-containing model bracoviruses: Microplitis bicoloratus bracovirus (MbBV) and Microplitis mediator bracovirus (MmBV). MbBV and MmBV nucleocapsids have discernable cap structures in two distal regions with relatively high electron density. Adjacent to the end-cap structures are two electron-lucent rings. Some nucleocapsids were uniformly electron-dense and had a distinctive helix-tail-like structure. Cryo-EM revealed inconsistent nucleocapsid diameters of 34-69.9nm in MbBV and 46-69.9nm in MmBV, and the largest observed cylindrical area length was expanded to 126nm.

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