Development of plate-type and tubular chemiluminescence immunoassay against African swine fever virus p72

文献类型： 外文期刊

第一作者： Miao, Chun

作者： Miao, Chun;Shao, Junjun;Yang, Sicheng;Wen, Shenghui;Ma, Yunyun;Gao, Shandian;Chang, Huiyun;Liu, Wei;Miao, Chun;Shao, Junjun;Yang, Sicheng;Wen, Shenghui;Ma, Yunyun;Gao, Shandian;Chang, Huiyun;Liu, Wei

作者机构：

关键词： African swine fever virus; p72-CLIA; p72-MPCLIA; Diagnosis

期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：3.9； 五年影响因子：4.9 ）

ISSN： 0175-7598

年卷期： 2024 年 108 卷 1 期

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收录情况： SCI

摘要： African swine fever (ASF) is a highly contagious and fatal viral disease that has caused huge economic losses to the pig and related industries worldwide. At present, rapid, accurate, and sensitive laboratory detection technologies are important means of preventing and controlling ASF. However, because attenuated strains of African swine fever virus (ASFV) are constantly emerging, an ASFV antibody could be used more effectively to investigate the virus and control the disease on pig farms. The isolation of ASFV-specific antibodies is also essential for the diagnosis of ASF. Therefore, in this study, we developed two chemiluminescence immunoassays (CLIAs) to detect antibodies directed against ASFV p72: a traditional plate-type blocking CLIA (p72-CLIA) and an automatic tubular competitive CLIA based on magnetic particles (p72-MPCLIA). We compared the diagnostic performance of these two methods to provide a feasible new method for the effective prevention and control of ASF and the purification of ASFV. The cut-off value, diagnostic sensitivity (Dsn), and diagnostic specificity (Dsp) of p72-CLIA were 40%, 100%, and 99.6%, respectively, in known background serum, whereas those of p72-MPCLIA were 36%, 100%, and 99.6%, respectively. Thus, both methods show good Dsn, Dsp, and repeatability. However, when analytical sensitivity was evaluated, p72-MPCLIA was more sensitive than p72-CLIA or a commercial enzyme-linked immunosorbent assay. More importantly, p72-MPCLIA reduced the detection time to 15 min and allowed fully automated detection. In summary, p72-MPCLIA showed superior diagnostic performance and offered a new tool for detecting ASFV infections in the future.

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