Precise pathogen quantification by CRISPR-Cas: a sweet but tough nut to crack

文献类型： 外文期刊

第一作者： Yao, Zhihao

作者： Yao, Zhihao;Li, Wanglu;He, Kaiyu;Wang, Hongmei;Xu, Xiahong;Wang, Liu;Yao, Zhihao;Xu, Yan;Wu, Qun;Wang, Liu

作者机构：

关键词： Pathogen; CRISPR-Cas; biosensor; quantification; isothermal amplification

期刊名称：CRITICAL REVIEWS IN MICROBIOLOGY （ 影响因子：5.1； 五年影响因子：7.5 ）

ISSN： 1040-841X

年卷期： 2025 年 51 卷 4 期

页码：

收录情况： SCI

摘要： Pathogen detection is increasingly applied in medical diagnosis, food processing and safety, and environmental monitoring. Rapid, sensitive, and accurate pathogen quantification is the most critical prerequisite for assessing protocols and preventing risks. Among various methods evolved, those based on clustered regularly interspaced short palindromic repeats (CRISPR)-associated proteins (Cas) have been developed as important pathogen detection strategies due to their distinct advantages of rapid target recognition, programmability, ultra-specificity, and potential for scalability of point-of-care testing (POCT). However, arguments and concerns on the quantitative capability of CRISPR-based strategies are ongoing. Herein, we systematically overview CRISPR-based pathogen quantification strategies according to the principles, properties, and application scenarios. Notably, we review future challenges and perspectives to address the of precise pathogen quantification by CRISPR-Cas. We hope the insights presented in this review will benefit development of CRISPR-based pathogen detection methods.

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