High-efficiency flavonoid deglycosylation of whole cells with surface display of α-L-rhamnosidase in Escherichia coli

文献类型： 外文期刊

第一作者： Yan, Cheng-Hai

作者： Yan, Cheng-Hai;Mei, Ruo-Xi;Tan, Lu;Li, Yi-Jiang-Cheng;Herman, Richard Ansah;Xu, Yan;Gong, Lu-Chan;Wang, Jun;Herman, Richard Ansah;Xu, Yan;Gong, Lu-Chan;Wang, Jun;Wang, Ding;Wang, Jun

作者机构：

关键词： alpha-L-rhamnosidase; Deglycosylation; Flavonoid; Surface display; Whole-cell catalyst

期刊名称：FOOD BIOSCIENCE （ 影响因子：5.9； 五年影响因子：6.1 ）

ISSN： 2212-4292

年卷期： 2025 年 65 卷

页码：

收录情况： SCI

摘要： Natural flavonoids exhibit diverse physiological effects and are used as functional factors, antioxidants and pigments in food as well as pharmaceutical and cosmetic applications. However, their poor solubility and stability, result in low bioavailability. Biomodification through deglycosylation effectively enhances their bioactivity. This study developed two surface display strategies in Escherichia coli, anchoring alpha-L-rhamnosidase RhaB1-Delta N to the cell surface using the anchoring protein Lpp-OmpA. Immunofluorescence and proteinase K sensitivity tests confirmed the successful construction of surface-displaying strains E. coli Lpp-OmpA-RhaB1-Delta N and E. coli Lpp-OmpA-SpyCatcher/RhaB1-Delta N-SpyTag. Enzymatic property studies revealed improved temperature and pH stability of the whole-cell catalysts with surface compared to free enzymes. After 6 catalytic cycles, E. coli Lpp-OmpA-RhaB1-Delta N retained higher activity than E. coli RhaB1-Delta N. In flavonoid hydrolysis, E. coli Lpp-OmpA-RhaB1-Delta N increased yields of isoquercitrin and kaempferol-3-O-glucuronide by 20.25% and 10.99%, respectively, compared with that in whole-cell catalyst with intracellular enzyme (p < 0.05). In addition, it should be noted that although the enzyme expression of E. coli Lpp-OmpA-SpyCatcher/RhaB1-Delta N-SpyTag is reduced, its hydrolysis ability of flavonoids is comparable to that of E. coli RhaB1-Delta N due to the efficient catalytic ability of extracellular enzymes. This study establishes reliable whole-cell catalysts with surface-displayed alpha-L-rhamnosidase for efficient natural flavonoid modification, with potential applications in the food and pharmaceutical industries.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序