Clostridium butyricum-fermented Chinese herbal medicine enhances the immunity by modulating the intestinal microflora of largemouth bass (Micropterus salmoides)

文献类型： 外文期刊

第一作者： Meng, Xiaolin

作者： Meng, Xiaolin;Cai, Huimin;Li, Heng;You, Fu;Jiang, Aixia;Zhang, Xindang;Zhang, Yanmin;Chang, Xulu;Yang, Guokun;Hu, Wenpan;Li, Keke;Meng, Xiaolin;Zhang, Xindang;Zhang, Yanmin;Chang, Xulu;Yang, Guokun;Zhou, Zhigang

作者机构：

关键词： Largemouth bass; F-CHM; Immunity; SCFAs; Intestinal microflora

期刊名称：AQUACULTURE （ 影响因子：5.135； 五年影响因子：5.125 ）

ISSN： 0044-8486

年卷期： 2023 年 562 卷

页码：

收录情况： SCI

摘要： Probiotic-fermented Chinese herbal medicine has been shown to stimulate immunity and modulate gut micro -biota. It has been considered to be an ideal alternative to antibiotics in a variety of species. In this study, Clostridium butyricum was used as a fermentation strain, while a mixture of licorice root, Astragalus membranaceus and Acanthopanax senticosus was used as a substrate for solid-state fermentation. The fermented product (F-CHM) was mixed with basic feed at levels of 0 (NC), 5% (S-5%), 10% (S-10%) and 20% (S-20%) to feed largemouth bass for 56-day. The results showed that no significant effect of F-CHM was observed on weight gain, but the resis-tance to A.hydrophila infection was improved. The activity of alkaline phosphatase and lysozyme in plasma was increased with F-CHM addition, and the content of malondialdehyde was significantly decreased. In the spleen, a higher expression of the inflammatory factor TGF-beta and apoptosis related genes (Caspase-3 and p53) was detected after the addition of F-CHM. With the addition of F-CHM, the midgut villus height of largemouth bass was significantly increased, and the expression of the intestinal barrier related genes, ZO-1 and Claudin-4 was upregulated. Furthermore, the concentration of short-chain fatty acids was increased in the S-20% F-CHM addition group. Sequencing results showed that the intestinal flora abundance and diversity were dramatically increased in the S-10% group. In addition, the development of harmful bacteria was restrained, and the colo-nization of beneficial flora was increased. Collectively, dietary F-CHM can be applied as a largemouth bass feed supplement to enhance fish immunity, regulate the intestinal flora and improve the ability of the host to protect against pathogenic bacteria.

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