Molecular characterization of the integrative and conjugative elements harbouring multidrug resistance genes in Glaesserella parasuis

文献类型： 外文期刊

第一作者： Che, Yongliang

作者： Che, Yongliang;Wua, Renjie;Li, Hongjie;Wang, Longbai;Wu, Xuemin;Chen, Qiuyong;Chen, Rujing;Zhou, Lunjiang;Che, Yongliang;Wang, Longbai;Wu, Xuemin;Chen, Qiuyong;Chen, Rujing;Zhou, Lunjiang

作者机构：

关键词： Glaesserella (Haemophilus) parasuis; Integrative and conjugative element (ICE); Multidrug resistance; ICEHpa1 family; rhs family

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.3； 五年影响因子：3.5 ）

ISSN： 0378-1135

年卷期： 2024 年 291 卷

页码：

收录情况： SCI

摘要： It is widely known that integrative and conjugative elements (ICEs) play an important role in the transmission of resistance genes and other exogenous genes. The present study aimed to characterize the three novel ICEs including ICEGpa76, ICEGpa44, and ICEGpa11, from Glaesserella parasuis. The ICEs from G. parasuis strains d76, Z44, and XP11 were predicted and identified by whole-genome sequencing (WGS) analysis, ICEfinder, and PCR. Characterization of G. parasuis strains carrying ICEs were determined by conjugation assay, antimicrobial susceptibility testing, WGS, phylogenetic analysis, and comparative sequence analysis.The WGS results showed that three ICEs from G. parasuis have a common genetic backbone belonging to characteristics ofthe ICEHpa1 family. The sequence comparison showed that the ICEHpa1 family has five hot spots (HSs) determined by IS6, IS110, and IS256. Moreover, two variable regions (VRs), VR1 and VR2 were determined by multidrug resistance genes and the rearrangement hotspot (rhs) family, respectively. VR1 consists of multidrug resistance genes, ISApl1s, and other accessory genes, while VR2 is composed of IS4, rhs family, transposase, and hypothetical protein genes. Conjugation experiments and MICs revealed that three ICEs could be transferred to G. parasuis strain IV52, indicating these three ICEs could be transmitted horizontally among G. parasuis strains. Additionally, the difference in resistance genes from ICEs might be due to the insertion function of the ISApl1s in VR1, and the rhs family in VR2 might evolve andthen be stably inherited in G. parasuis. These results further elucidated the transmission mechanism of exogenous genes in G. parasuis.

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