A rapid detection of tomato yellow leaf curl virus using recombinase polymerase amplification-lateral flow dipstick assay

文献类型： 外文期刊

第一作者： Zhou, Y.

作者： Zhou, Y.;Liu, M.;Zhang, W.;Yan, J. Y.;Zheng, H. Y.;Jiang, D. M.

作者机构：

关键词： field detection; lateral flow dipstick; recombinase polymerase amplification; tomato yellow leaf curl virus

期刊名称：LETTERS IN APPLIED MICROBIOLOGY （ 影响因子：2.813； 五年影响因子：3.086 ）

ISSN： 0266-8254

年卷期： 2022 年 74 卷 5 期

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收录情况： SCI

摘要： Tomato yellow leaf curl disease which is caused by Tomato yellow leaf curl virus (TYLCV) is economically important and a widely spread tomato disease in China. Rapid and accurate detection methods are important in the control TYLCV. Here, a rapid method was developed to identify TYLCV on the basis of recombinase polymerase amplification (RPA) that can be visualized in 5 min using lateral flow dipsticks. The sensitivity and the specificity of this method were evaluated. This method can detect 0 center dot 5 pg DNA after 30 min at 37 degrees C without any expensive instrumentation. In addition, it showed higher sensitivity than a PCR method when purified DNA was used. Moreover, the TYLCV was specifically detected, whereas other viruses infecting tomato produced negative results. The crude tomato extracts used in this assay has potential application in minimally equipped plant clinic laboratories. This method will facilitate the early and rapid detection of TYLCV for the timely application of control measures.

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