Study on the anti-hyperuricemic bioactivity and chemical components of Sterculiae lychnophorae Semen

文献类型： 外文期刊

第一作者： Lv, Shen-Ming

作者： Lv, Shen-Ming;Liao, Zhi-Xin;Lv, Shen-Ming;Zhang, Meng-Qi;Chen, Jia-Shu;Sun, Jin-Yue;Liu, Chao;Chen, Ying-Ying;Guo, Xu;Liu, Jian-Shu;Wang, Mu-Xuan

作者机构：

关键词： Sterculiae lychnophorae Semen; Anti-hyperuricemia; Xanthine oxidase; Anti-inflammation

期刊名称：JOURNAL OF FUNCTIONAL FOODS （ 影响因子：5.223； 五年影响因子：5.178 ）

ISSN： 1756-4646

年卷期： 2022 年 95 卷

页码：

收录情况： SCI

摘要： Sterculiae lychnophorae Semen is an edible and medicinal resource and is widely used in functional foods in China. In the previous study, the S. lychnophorae ' function of anti-hyperuricemia was found, but its main functional ingredients have not been clarified. In this study, the major functional ingredients of S. lychnophorae with antihyperuricemic effect were determined under activity guided screening through the intervention for xanthine oxidase (XO) enzymatic reaction in vitro. The results showed EtOAc fraction (EF) of S. lychnophorae had the strongest inhibitory activity among all fractions with the inhibition rate 94.94%. Since hyperuricemia could trigger inflammation reaction, the anti-inflammation effect of mainly functional ingredient (EF) was also researched through a cell inflammation model established by using uric acid (UA) to stimulate HK-2 cells. The results showed that the expression levels of inflammatory cytokines IL-6, IL-1 beta and TNF-alpha were decreased after treatment with EF. On the meanwhile, molecular mechanistic studies revealed that EF could downregulate the phosphorylation levels of PI3K, AKT, I kappa B alpha, NF-kappa B proteins in HK-2 cells. These results showed that EF could potentially lower UA level and combat inflammation by inhibiting the PI3K/AKT/NF-kappa B signal pathway. Furthermore, the chemical components of EF were also clarified. As a result, two new compounds, ethyl 2, 3dihydro-1, 4-benzodioxin-6-carboxylate (1) and ethyl 3-(butyryloxy)-4-hydroxybenzoate (2), together with 15 known compounds were identified from EF by extensive NMR experiments and comparison with the data reported in the literature. The XO inhibitory effects of all the compounds were analyzed. With the determination of the anti-hyperuricemic mechanism and chemical component of EF, it can be used as a raw material to develop functional foods against hyperuricemia in the future.

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