Lipid A-modified Escherichia coli can produce porcine parvovirus virus-like particles with high immunogenicity and minimal endotoxin activity

文献类型： 外文期刊

第一作者： Shen, Xuegang

作者： Shen, Xuegang;Yang, Yong-Bo;Gao, Yanfei;Wang, Shujie;Wang, Haiwei;Sun, Mingxia;Meng, Fandan;Tang, Yan-Dong;Tu, Yabin;An, Tong-Qing;Cai, Xue-Hui;Kong, Qingke;An, Tong-Qing;Yang, Yong-Bo;Cai, Xue-Hui

作者机构：

关键词： Porcine parvovirus; Virus-like particle; Minimal endotoxin activity; Escherichia coli; Lipid A

期刊名称：MICROBIAL CELL FACTORIES （ 影响因子：4.3； 五年影响因子：5.5 ）

ISSN：

年卷期： 2024 年 23 卷 1 期

页码：

收录情况： SCI

摘要： Background A cost-effective Escherichia coli expression system has gained popularity for producing virus-like particle (VLP) vaccines. However, the challenge lies in balancing the endotoxin residue and removal costs, as residual endotoxins can cause inflammatory reactions in the body. Results In this study, porcine parvovirus virus-like particles (PPV-VLPs) were successfully assembled from Decreased Endotoxic BL21 (BL21-DeE), and the effect of structural changes in the lipid A of BL21 on endotoxin activity, immunogenicity, and safety was investigated. The lipopolysaccharide purified from BL21-DeE produced lower IL-6 and TNF-alpha than that from wild-type BL21 (BL21-W) in both RAW264.7 cells and BALB/c mice. Additionally, mice immunized with PPV-VLP derived form BL21-DeE (BL21-DeE-VLP) showed significantly lower production of inflammatory factors and a smaller increase in body temperature within 3 h than those immunized with VLP from BL21-W (BL21-W-VLP) and endotoxin-removed VLP (ReE-VLP). Moreover, mice in the BL21-DeE-VLP immunized group had similar levels of serum antibodies as those in the BL21-W-VLP group but significantly higher levels than those in the ReE-VLP group. Furthermore, the liver, lungs, and kidneys showed no pathological damage compared with the BL21-W-VLP group. Conclusion Overall, this study proposes a method for producing VLP with high immunogenicity and minimal endotoxin activity without chemical or physical endotoxin removal methods. This method could address the issue of endotoxin residues in the VLP and provide production benefits.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序