The immunogenic maturation of goat monocyte-derived dendritic cells and upregulation of toll-like receptors by five antigens of Haemonchus contortus in-vitro

文献类型： 外文期刊

第一作者： Hasan, Muhammad Waqqas

作者： Hasan, Muhammad Waqqas;Haseeb, Muhammad;Ehsan, Muhammad;Gadahi, Javaid Ali;Wang, Qiangqiang;Memon, Muhammad Ali;Aleem, Muhammad Tahir;Lakho, Shakeel Ahmed;Yan, Ruo Feng;Xu, Li Xin;Song, Xiao Kai;Li, Xiangrui;Ehsan, Muhammad;Gadahi, Javaid Ali

作者机构：

关键词： Haemonchus contortus; Immuno-stimulatory; antigens; Goat monocytes; Dendritic cell functions; Activation of the immune response

期刊名称：RESEARCH IN VETERINARY SCIENCE （ 影响因子：2.534； 五年影响因子：2.382 ）

ISSN： 0034-5288

年卷期： 2021 年 136 卷

页码：

收录情况： SCI

摘要： Previously, it was found that several proteins of Haemonchus contortus were involved in the stimulation of the host immune system. However, the information about the selection of superlative antigens with immunogenic efficacies on host DCs is lacking. In the current study, the stimulatory effects of five recombinant proteins (elongation factor-1 alpha, arginine kinase, ES-15, ES-24, and ADP-ribosylation factor 1) of H. contortus on the maturation of goat monocyte-derived dendritic cells (md-DCs) were reported. Recombinant proteins were purified separately in E. coli expression and incubated with isolated goat peripheral blood mononuclear cells (PBMC). Immunofluorescence assay (IFA) results confirmed the binding of these molecules to the md-DC's surface as compared to control groups. In the flow cytometry analysis, recombinant proteins induced md-DC stimulation via the up-regulation of the expression of the costimulatory molecule (CD80) and MHC-II. Quantitative RT-PCR data showed a significant increase in the expression of specific genes of the WNT and toll-like receptor (TLR) signaling pathways. The result of ELISA indicated the higher levels of cytokine (IL-10, IL-12, IFN-gamma, and TNF-alpha) secretion in the md-DC compared to the negative (pET-32a His-Tag) and blank (PBS) control groups. The data gives valuable support in the selection of potential antigens for future studies on the immunomodulation of the host against the infection of H. contortus.

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