Three-step evolutionary enhanced capillary electrophoresis-SELEX for aptamer selection of exosome vesicles

文献类型： 外文期刊

第一作者： Zhao, Liping

作者： Zhao, Liping;Luan, Yunxia;Zhao, Liping;Li, Linsen;Zhao, Yi;Qu, Feng;Yang, Ge;Zhu, Chao

作者机构：

关键词： NK exosome; CE-SELEX; Selection strategy; Aptamer

期刊名称：TALANTA （ 影响因子：6.1； 五年影响因子：5.4 ）

ISSN： 0039-9140

年卷期： 2024 年 267 卷

页码：

收录情况： SCI

摘要： As various disease biomarkers, drugs/biomolecules carriers and physiological function regulator, exosomes play significant roles in various physiological and pathological process. Thus, the purification and detection of some exosome are of great significance, which make it necessary for the development of the recognition elements. Aptamers with high affinity and specificity exhibited well in the above areas. In this work, a new three-step evolutionary enhanced strategy based on capillary electrophoresis (CE)-SELEX was proposed for effective aptamer selection of exosome vesicles for the first time. Natural killer cells (NK) exosome was used as the model target. And the aptamer could be obtained in five round CE selection within three steps evolution including precise nucleic acid sequence convergence (with NK exosome specific proteins as targets in round 1st and 3rd), affinity evolution (with NK exosome as target in round 2nd and 4th) and specific evolution (negative selection in round 5th). Arising from the compatibility of CE-SELEX for exosome vesicles aptamer selection, and the proposed strategy, aptamer against NK exosome had been selected with good affinity (KD value of 27.6 nM) and excellent specificity. The as proposed CE-SELEX strategy paves a novel way for recognition element selection of exosome vesicles.

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