Duck compound probiotics fermented diet alters the growth performance by shaping the gut morphology, microbiota and metabolism

文献类型： 外文期刊

第一作者： Li, Zhaolong

作者： Li, Zhaolong;Li, Cuiting;Lin, Fengqiang;Yan, Lu;Wu, Huini;Zhou, Haiou;Guo, Qing;Huang, Yu;Lin, Binbin;Xie, Bilin;Xu, Yijuan;Lin, Zhimin;Liu, Wenjin

作者机构：

关键词： fermented diet; duck; compound probiotics; growth performance

期刊名称：POULTRY SCIENCE （ 影响因子：4.4； 五年影响因子：4.4 ）

ISSN： 0032-5791

年卷期： 2024 年 103 卷 6 期

页码：

收录情况： SCI

摘要： Dietary absorption and digestion are in fluenced by the microbiota, morphology, and digestive enzymes of intestines, and fermentation is a popular and effective technique to enhance animal rearing growth performance. This study aims to explore the pivotal role of Muscovy duck probiotics fermented feedstuff ( FF ) in altering the growth performance by reshaping gut morphology, microorganisms and metabolism. The findings showed that FF considerably raised the levels of fatty acids (FA) and small peptides (7-19AA) in the diet. Further feeding trial data reveals that FF greatly increased the Muscovy duck average daily gain ( ADG ) but had no effect on their daily feed intake ( DFI ), and the FCR signi ficantly dropped ( P < 0.05). Additionally, it was evident that FF improved the integrity of the intestinal mucosa in Muscovy duck by increasing villus height, villus height -to -crypt depth ratio, and lowering crypt depth. Then, in comparison to the control group ( NC ), there was a signi ficant increase in the gene expression of the mucosal tight junction proteins Occludin, Claudin-1, and Zo-1 in the intestine of Muscovy duck. Additionally, there was higher expression of the mucosal transport channels SGLT-1, PepT1, AQP2, AQP3, and AQP10 in the similarly colon site, jejunum, and duodenum. Furthermore, in AB-PAS/PAS-stained duodenum, jejunum, ileum, and similarly colon site, FF markedly increased relative mucus output and goblet cells while decreasing epithelial cell apoptosis. Following 16S sequencing data indicated that the intestinal microbiota was altered and the diversity and richness of gut microbes was greatly enhanced by FF. Particularly, the boost of core probiotics, such as Rothia of duodenum, Limosilactobacillus and Lentilactobacillus of jejunum, Lactococcus and Rothia of ileum, Ligilactobacillus and Entocuccus of similarly colon site, Gallibacterium of caecum. And reduced potentially pathogenic bacteria ( Campylobacter, Prevotellaceae, Clostridia-vadinBB60, and Oscillospira ). Nontargeted metabolomics assay for intestinal content con firmed an increased organic acids (oxidanesulfonic acid, cholic acid, gallic acid, coumaric acid, pipecollc acid, 13s-hydroxyoctadecadienoic acid) and glycosides metabolites (5-hydroxydantrolene, 3-hydroxyguanfacine glucuronide, acetylleucine, astragalin, xanthosine, taxiphylin, sinapine, denudatine, penylalanyl-tyrosine and phenylalanyl-valine). These findings demonstrated that FF, a viable option to improve Muscovy duck growth performance through reconstructed intestinal morphology, microorganisms, and metabolism, subsequently promoted the gut health and increased diet digestion and absorption. The study that is being presented offers scienti fic proof that FF might be a useful strategy for improving Muscovy duck growth performance.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序