The Impact of FBN1-α5β1 Axis in Fibro/Adipogenic Progenitor Cells (FAPCD9-) on Intramuscular Fat Content in Pigs

文献类型： 外文期刊

第一作者： Tong, Xian

作者： Tong, Xian;Zhu, Qi;Duo, Tianqi;Liang, Ziyun;Zhang, Chong;Cai, Shufang;Wang, Xiaoyu;Liu, Yihao;Li, Yongpeng;Liu, Xiaohong;He, Zuyong;Chen, Yaosheng;Mo, Delin;Hu, Bin;Zeng, Jianhua

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关键词： intramuscular fat; scRNA-seq; pig; Fibro/Adipogenic Progenitor Cells; FBN1-Integrin alpha 5 beta 1axis

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.1； 五年影响因子：6.3 ）

ISSN： 0021-8561

年卷期： 2024 年 72 卷 16 期

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收录情况： SCI

摘要： Intramuscular fat (IMF) plays a crucial role in enhancing meat quality, enriching meat flavor, and overall improving palatability. In this study, Single-cell RNA sequencing was employed to analyze the longissimus dorsi (LD) obtained from Guangdong small-ear spotted pigs (GDSS, with high IMF) and Yorkshire pigs (YK, with low IMF). GDSS had significantly more Fibro/Adipogenic Progenitor (FAPs), in which the CD9 negative FAPs (FAP(CD9-)) having adipogenic potential, as demonstrated by in vitro assays using cells originated from mouse muscle. On the other hand, Yorkshire had more fibro-inflammatory progenitors (FIPs, marked with FAP(CD9+)), presenting higher expression of the FBN1-Integrin alpha 5 beta 1. FBN1-Integrin alpha 5 beta 1 could inhibit insulin signaling in FAP(CD9-), suppressing adipogenic differentiation. Our results demonstrated that fat-type pigs possess a greater number of FAP(CD9-), which are the exclusive cells in muscle capable of differentiating into adipocytes. Moreover, lean-type pigs exhibit higher expression of FBN1-Integrin alpha 5 beta 1 axis, which inhibits adipocyte differentiation. These results appropriately explain the observed higher IMF content in fat-type pigs.

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