Composition and temporal dynamics of the phytoplankton community in Laizhou Bay revealed by microscopic observation and rbcL gene sequencing

文献类型： 外文期刊

第一作者： Zhang, Huihui

作者： Zhang, Huihui;Wang, Nan;Zhang, Di;Ding, Xiaokun;Tian, Jinghuan;Jiang, Tao;Wang, Fei;Li, Bin;Xu, Shiji;Xie, Yixuan;Cui, Zhengguo

作者机构：

关键词： Algal blooms; Community composition; Laizhou Bay; Microscopic observation; Phytoplankton; rbcL

期刊名称：MARINE ENVIRONMENTAL RESEARCH （ 影响因子：3.2； 五年影响因子：3.3 ）

ISSN： 0141-1136

年卷期： 2024 年 202 卷

页码：

收录情况： SCI

摘要： Laizhou Bay, a major breeding ground for economic marine organisms in the northern waters of China, is facing rapid environmental degradation. In this study, field surveys in this area were conducted in the spring, summer, and autumn of 2020. Microscopic observation and RuBisCO large subunit (rbcL) gene analysis were employed to understand the community structure and temporal dynamics of phytoplankton. The phytoplankton community structures detected by the two methods showed significant differences. Microscopic observation revealed the dominance of dinoflagellates in spring that shifted to the dominance of diatoms in summer and autumn. However, rbcL gene sequencing consistently identified diatoms as dominant throughout all three seasons, with their relative abundance showing an increasing trend. Conversely, the relative abundance of the second- and thirdmost abundant taxa, namely, haptophytes and ochrophytes, decreased as the seasons transitioned. rbcL gene sequencing annotated more species than microscopy. It could detect haptophytes and cryptophytes, which were overlooked by microscopy. In addition, rbcL gene sequencing detected a remarkable amount of Thalassiosira profunda, which was previously unidentified in this sea area. However, it appeared to underestimate the contribution of dinoflagellates considerably, with most taxa being only identified through microscopic identification. The two methods jointly identified 28 harmful algal bloom taxa with similar detection quantities but substantial differences in species composition. Phytoplankton communities were influenced by temperature, salinity, and nutrients. The results of this work suggest that a combination of multiple techniques is necessary for a comprehensive understanding of phytoplankton.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序