Gold core @ platinum shell nanozyme-mediated magnetic relaxation switching DNA sensor for the detection of Listeria monocytogenes in chicken samples

文献类型： 外文期刊

第一作者： Wu, Zijing

作者： Wu, Zijing;Huang, Chenxi;Dong, Yongzhen;Zhao, Binjie;Chen, Yiping;Chen, Yiping;Wu, Zijing;Huang, Chenxi;Dong, Yongzhen;Chen, Yiping;Chen, Yiping

作者机构：

关键词： Listeria monocytogenes; Au@Pt nanozymes; Magnetic relaxation switching; Mn (VII)/Mn (II) conversion; Hydrogen peroxide; DNA hybridization

期刊名称：FOOD CONTROL （ 影响因子：6.652； 五年影响因子：6.498 ）

ISSN： 0956-7135

年卷期： 2022 年 137 卷

页码：

收录情况： SCI

摘要： Herein, an Au@Pt nanozyme-mediated magnetic relaxation switching (MRS) DNA biosensor has been developed for the rapid detection of Listeria monocytogenes. Au@Pt nanoparticle was with enhanced stability and high peroxidase activity for decomposing the hydrogen peroxide. After DNA hybridization and magnetic separation, the conjugates of MNP180-probe(1)-L. monocytogenes DNA-probe2-Au@Pt could adjust the hydrogen peroxide mediated Mn(VII)/Mn(II) conversion, and thus leading to the change of the transverse relaxation time. The amount of Au@Pt was directly proportional to the concentration of L. monocytogenes. The Au@Pt nanozyme and the hydrogen peroxide-mediated Mn(VII)/Mn(II) signal conversion system contributed to the sensitivity and stability of this sensor. This sensor enabled the detection of L. monocytogenes with a LOD of 30 CFU/mL, which was 33-folds enhancement compared with that of enzyme-linked immunosorbent assay, providing a promising platform to trace the foodborne pathogen in the complex food matrix.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序