The GABA-modulated energy metabolism reconfiguration positively regulates cotton (Gossypium hirsutum L.) responses during post-waterlogging recovery

文献类型： 外文期刊

第一作者： Wang, Li

作者： Wang, Li;Zheng, Saiya;Li, Leyue;Liu, Xuanxuan;Feng, Liuchun;Liu, Xinyan;Huang, Qingqing;Zhang, Yibin;Yan, Gentu;Wang, Ning;Ma, Chunmei;Yan, Gentu;Wang, Ning

作者机构：

关键词： Cotton; RNA-sequencing; Widely targeted metabolomics; Post-waterlogging stress; Alternative energy metabolism; Reactive oxygen species

期刊名称：INDUSTRIAL CROPS AND PRODUCTS （ 影响因子：6.2； 五年影响因子：6.2 ）

ISSN： 0926-6690

年卷期： 2025 年 231 卷

页码：

收录情况： SCI

摘要： Plant response to waterlogging is well studied, but the understanding of plant responses during post-waterlogging recovery remains limited. In this study, we show that the waterlogging-sensitive (Z4847) and waterlogging-tolerant (ZM9001) cotton varieties have different rates of post-waterlogging recovery that correlate with waterlogging tolerance. A comparative transcriptome and metabolome analysis of the roots from two cotton varieties during recovery processes indicated significant inhibition of the abundance of genes and metabolites related to energy metabolism in both varieties during post-waterlogging. The results also showed that ZM9001 plants maintained higher carbon flows from glycolysis to the first reaction of the TCA cycle, leading to more energy production and restoration of the root activity during recovery processes. The KEGG enrichment analysis based on the combined transcriptomics and metabolomics between ZM9001 and Z4847 during post-waterlogging displayed a close connection between GABA metabolism and the glycolysis-citrate cycle. Thus we integrated physiological and multi-omics approaches and showed that the induced elevation of the GABA levels in the ZM9001 plants during recovery processes is critical for the root function restoration by increasing the energy through the GABA shunt activation. Consistent with this, the exogenous application of GABA enhanced the root ATP content and post-waterlogging tolerance of cotton, whereas the loss of GABA synthesis by silencing GhGAD1 showed increased sensitivity to post-waterlogging. Subsequently, elevated GABA levels could also provide better redox homeostasis by enhancing the antioxidant enzyme activities during post-waterlogging recovery. These findings indicate that the energy metabolism reconfiguration via re-activating GABA shunt participates in seedling survival after waterlogging stress.

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