Preparation and immunoactivity of sulfated glucan from Saccharomyces cerevisiae liposomes

文献类型： 外文期刊

第一作者： Tao, Yijiong

作者： Tao, Yijiong;Huang, Linjie;Li, Zhaolong;Li, Jiayi;Tang, Qi;Chen, Kai;Zhang, Lifang;Fei, Chenzhong;Liu, Yinchun;Wang, Mi

作者机构：

关键词： Sulfated glucan from Saccharomyces cerevisiae; liposome; immuno-activity; proliferation of chicken spleen lymphocytes; cytokine concentrations

期刊名称：JOURNAL OF LIPOSOME RESEARCH （ 影响因子：4.3； 五年影响因子：5.0 ）

ISSN： 0898-2104

年卷期： 2025 年

页码：

收录情况： SCI

摘要： This study optimized the preparation conditions of sulfated glucan from Saccharomyces cerevisiae liposomes (SGSCL) and evaluated its effect on immune activity. SGSCL was prepared using the reverse evaporation method, and its immune activity was assessed by measuring the proliferation of chicken spleen lymphocytes, hemagglutination inhibition (HI) antibody titers, and serum cytokine concentrations in chickens vaccinated with the Newcastle disease (ND) vaccine. The optimal preparation conditions were a phospholipid-to-cholesterol mass ratio of 5.4:1, a phospholipid-to-SGSC mass ratio of 10:1, and a rotary evaporation temperature of 40 degrees C. The average encapsulation efficiency (EE) was 63.92%, whereas the mean particle size, polymer dispersity index (PDI), and zeta potential were 90.39 +/- 1.71 nm, 0.203 +/- 0.004, and -41.13 +/- 1.05 mV, respectively. SGSCL significantly promoted the proliferation of chicken spleen lymphocytes, splenic T and B lymphocytes at concentrations of 100-800 mu g/mL, 800 mu g/mL and 200-800 mu g/mL in vitro. The best proliferative effect on splenic lymphocytes were at 400 mu g/mL, 800 mu g/mL, and 800 mu g/mL. In vivo, on days 7 and 14, HI antibody titers in the SGSCL-H, SGSCL-M, and SGSCL-L groups were significantly greater than those in the VC group. The serum antibody titers in the SGSCL-H and SGSCL-M groups were significantly or numerically elevated compared to the VC group at all time points post-vaccination. The IL-2, IL-6, IL-4, and IFN-gamma concentrations in the SGSCL-H group were significantly higher than that in the VC group on D28 and D35. These findings suggest that SGSCL could serve as a novel vaccine diluent or immune adjuvant.

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