Siglec-15 is a putative receptor for porcine epidemic diarrhea virus infection

文献类型： 外文期刊

第一作者： Feng, Zhihua

作者： Feng, Zhihua;Chen, Jianxin;Chen, Qi;Feng, Zhihua;Chen, Jianxin;Chen, Qi;Feng, Zhihua;Fu, Yajuan;Yang, Sheng;Zhao, Heng;Lin, Minhua;Liu, Chuancheng;Huang, Weili;He, Xinyan;Chen, Yao;Shen, Yangkun;Chen, Qi;Feng, Zhihua;Fu, Yajuan;Yang, Sheng;Zhao, Heng;Lin, Minhua;Liu, Chuancheng;Huang, Weili;He, Xinyan;Chen, Yao;Shen, Yangkun;Chen, Qi;Li, Zhaolong

作者机构：

关键词： Swine alpha-coronaviruses; Host factor; Mouse model; Antibody drug; Cross-species transmission

期刊名称：CELLULAR AND MOLECULAR LIFE SCIENCES （ 影响因子：6.2； 五年影响因子：7.7 ）

ISSN： 1420-682X

年卷期： 2025 年 82 卷 1 期

页码：

收录情况： SCI

摘要： Porcine epidemic diarrhea virus (PEDV) has caused significant losses in the pork industry, but the mechanism of PEDV infection is still unclear. On the basis of our RNA-Seq data and due to the potential role of sialic acid as a coreceptor, we investigated the function of sialic acid-binding Ig-like lectin 15 (Siglec-15) to determine its role as a receptor in PEDV infection. We found that Siglec-15 enhances PEDV infection by promoting viral adsorption to host cells. Coimmunoprecipitation and immunofluorescence assays revealed that Siglec-15 binds to the S1 subunit and M protein of PEDV. PEDV infectivity was significantly reduced in Siglec-15 knockout mice. In addition, we developed a monoclonal antibody targeting Siglec-15 that can effectively inhibit PEDV infection both in vitro and in vivo. Overall, our study suggests that Siglec-15 may be a receptor for PEDV infection, which is important for related mechanistic studies and reveals a novel target for anti-PEDV therapeutic development.

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