Trichinella spiralis excretory/secretory antigens ameliorate porcine epidemic diarrhea virus-induced mucosal damage in porcine intestinal oganoids by alleviating inflammation and promoting tight junction

文献类型： 外文期刊

第一作者： Liu, Yinju

作者： Liu, Yinju;Tan, Jinlong;Zhang, Nianzhang;Qu, Zigang;Li, Wenhui;Wu, Yaodong;Yin, Hong;Liu, Guangliang;Fu, Baoquan;Tan, Jinlong;Yin, Hong;Fu, Baoquan

作者机构：

关键词： Porcine intestinal organoid; Trichinella spiralis excretory/secretory; antigens; Porcine epidemic diarrhea virus; Inflammation; Mucosal lesion

期刊名称：INTERNATIONAL JOURNAL FOR PARASITOLOGY （ 影响因子：3.2； 五年影响因子：3.9 ）

ISSN： 0020-7519

年卷期： 2025 年 55 卷 3-4 期

页码：

收录情况： SCI

摘要： Trichinella spiralis and porcine epidemic diarrhea virus (PEDV) are two infectious swine pathogens. Parasite excretory/secretory antigens play critical roles in various disease processes. To explore the coexistence mechanism of two pathogens infecting the same host, the intestinal organoid was utilized to reproduce these biological processes. In this study, we investigated the effects of T. spiralis excretory/secretory antigens (TsES) on PEDV-induced inflammatory regulation, lesion recovery, and mucosal barrier repair in porcine intestinal organoids. The results showed that PEDV effectively infected the porcine intestinal organoids. Next, TsES inhibited pro-inflammatory cytokines and increased the antiinflammatory cytokines produced by PEDV-infected porcine intestinal organoids. Further, fourdimensional (4D) label-free quantitative proteomics and western blotting confirmed that TsES regulate the inflammation caused by PEDV infection through the nuclear factor kappa-B (NF-KB) pathway. In addition, TsES promoted cell proliferation, inhibited apoptosis, and reduced PEDV-induced lesions in intestinal organoids. The elevated secretory immunoglobulin A (sIgA) levels caused by PEDV infection were downregulated by TsES treatment in intestinal organoids. TsES treatment reversed the mucosal barrier damage caused by PEDV infection in intestinal organoids. Finally, PEDV replication increased after TsES treatment in organoids. We highlight the potential of TsES to ameliorate PEDV-induced inflammation, mucosal lesions, and barrier damage in porcine intestinal organoids. TsES also contribute to PEDV replication. This study presents a novel research model for research on host-virus-parasite interactions, while also providing a theoretical foundation to consider parasite derivatives as a potential adjunctive therapy for intestinal inflammation. (c) 2024 Australian Society for Parasitology. Published by Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.

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