Characterisation and preliminary functional analysis of N-acetyltransferase 13 from Schistosoma japonicum

文献类型： 外文期刊

第一作者： Tang, Yalan

作者： Tang, Yalan;Zhou, Kerou;Guo, Qingqing;Chen, Cheng;Jia, Jing;Guo, Qinghong;Lu, Ke;Li, Hao;Fu, Zhiqiang;Liu, Jinming;Lin, Jiaojiao;Hong, Yang;Tang, Yalan;Zhou, Kerou;Guo, Qingqing;Chen, Cheng;Jia, Jing;Guo, Qinghong;Lu, Ke;Li, Hao;Fu, Zhiqiang;Liu, Jinming;Lin, Jiaojiao;Hong, Yang;Yu, Xingang

作者机构：

关键词： Schistosoma japonicum; SjNAT13; N-acetyltransferase; RNAi; Development; Reproduction; Schistosome

期刊名称：BMC VETERINARY RESEARCH （ 影响因子：2.741； 五年影响因子：2.955 ）

ISSN：

年卷期： 2021 年 17 卷 1 期

页码：

收录情况： SCI

摘要： Background N-acetyltransferase 13 (NAT13) is a probable catalytic component of the ARD1A-NARG1 complex possessing alpha (N-terminal) acetyltransferase activity. Results In this study, a full-length complementary DNA (cDNA) encoding Schistosoma japonicum NAT13 (SjNAT13) was isolated from schistosome cDNAs. The 621 bp open reading frame of SjNAT13 encodes a polypeptide of 206 amino acids. Real-time PCR analysis revealed SjNAT13 expression in all tested developmental stages. Transcript levels were highest in cercariae and 21-day-old worms, and higher in male adult worms than female adult worms. The rSjNAT13 protein induced high levels of anti-rSjNAT13 IgG antibodies. In two independent immunoprotection trials, rSjNAT13 induced 24.23% and 24.47% reductions in the numbers of eggs in liver. RNA interference (RNAi) results showed that small interfering RNA (siRNA) Sj-514 significantly reduced SjNAT13 transcript levels in worms and decreased egg production in vitro. Conclusions Thus, rSjNAT13 might play an important role in the development and reproduction of schistosomes.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序