Establishment and characterization of a novel human gallbladder cancer cell line, GBC-X1

文献类型： 外文期刊

第一作者： Chai, Changpeng

作者： Chai, Changpeng;Li, Lu;Miao, Long;Zhang, Bo;Wang, Zhengfeng;Luo, Wei;Xu, Hao;Chai, Changpeng;Tang, Huan;Chen, Tingting;Su, Yuanhui;Miao, Long;Wang, Zhengfeng;Zhang, Hui;Zhou, Wence;Miao, Xin;Miao, Xin;Xu, Hao;Zhang, Hui;Zhou, Wence;Xu, Hao

作者机构：

关键词： Gallbladder cancer; Cell line; Establishment; Drug resistance

期刊名称：SCIENTIFIC REPORTS （ 影响因子：3.9； 五年影响因子：4.3 ）

ISSN： 2045-2322

年卷期： 2024 年 14 卷 1 期

页码：

收录情况： SCI

摘要： In this study, we successfully established a novel gallbladder cancer cell line, designated as GBC-X1, derived from a primary tumor of a gallbladder cancer patient. By comprehensively analyzing the cell line's phenotype, molecular characteristics, biomarkers, and histological characteristics, we confirmed that GBC-X1 serves as a valuable model for investigating the pathogenesis of gallbladder cancer and developing therapeutic agents. GBC-X1 has been continuously cultured for one year, with over 60 stable passages. Morphologically, GBC-X1 exhibits typical features of epithelial tumors. The population doubling time of GBC-X1 is 32 h. STR analysis validated a high consistency between GBC-X1 and the patient's primary tumor. Karyotype analysis revealed an abnormal hypertetraploid karyotype for GBC-X1, characterized by representative karyotypes of 98, XXXX del (4) p (12) del (5) p (21) der (10). Under suspension culture conditions, GBC-X1 efficiently forms tumor balls, while subcutaneous inoculation of GBC-X1 cells into NXG mice leads to xenograft formation with a rate of 80%. Drug sensitivity testing demonstrated that GBC-X1 is resistant to oxaliplatin and sensitive to 5-FU, gemcitabine, and paclitaxel. Immunohistochemistry revealed positive expression of CK7, CK19, E-cadherin, MMP-2, CD44, SOX2, and TP53 in GBC-X1 cells, weak positive expression of Vimentin, and a Ki67 positive rate of 35%. Our research highlights GBC-X1 as a novel gallbladder cancer cell line and emphasizes its potential as an effective experimental model for investigating the pathogenesis of gallbladder cancer and drug development.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序