Dynamic Pollen-Stigma Coordination in Dendrobium Hybridization: A Strategy to Maximize Fruit Set and Hybrid Seed Viability

文献类型： 外文期刊

第一作者： Wu, Qian

作者： Wu, Qian;Qian, Yanbing;Guan, Ao;Yue, Yan;Li, Zongyan;Dunn, Bruce;Yang, Jianwei;Yi, Shuangshuang;Liao, Yi;Yin, Junmei;Yin, Junmei

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关键词： Dendrobium; interspecific hybridization; developmental traits; pollen; sigma

期刊名称：HORTICULTURAE （ 影响因子：3.0； 五年影响因子：3.2 ）

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年卷期： 2025 年 11 卷 5 期

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收录情况： SCI

摘要： This study investigated dynamic pollen-stigma coordination to optimize interspecific hybridization in Dendrobium using D. 'Burana Jade' as the maternal parent and eight wild species as pollen donors. Stigma receptivity was comprehensively evaluated using a multi-indicator approach, including morphological characterization (crystal secretion and bulging papillae), histochemical benzidine-H2O2 staining, and enzymatic activity profiling (esterase and superoxide dismutase). Concurrently, pollen viability was assessed through TTC testing coupled with ultrastructural observations. Results identified a critical synchronization window: pollen viability peaked at 1-3 days post anthesis (DPA) or during the mid-anthesis phase, while stigmas exhibited maximal receptivity when secretory activity and antioxidant enzyme levels significantly increased. Using stage-specific pollination criteria, 8.4% of crosses (8/95) produced viable fruits, outperforming empirical methods by 2.8-fold. D. 'Burana Jade' showed cross-compatibility with four Dendrobium species (D. aphyllum, D. chrysotoxum, D. hercoglossum, D. thyrsiflorum), with D. thyrsiflorum hybrids achieving 54.81% embryogenesis and 22.38% germination. Three compatible combinations germinated successfully in vitro within 45-55 days on 1/4 MS medium supplemented with 20 g/L sucrose, 1 g/L tryptone, 180 mL/L coconut water, and 2.2 g/L Phytagel. Our findings establish that synchronizing pollen viability windows with stigma receptivity phases significantly enhances fruit set and hybrid seed viability, providing a phenology-driven strategy to overcome reproductive barriers in orchid breeding programs. This study provides key physiological criteria for Dendrobium hybridization, though their applicability to other orchids needs validation. Future multi-omics studies should explore cross-species compatibility mechanisms.

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