Cold tolerance identification of nine Rosa L. materials and expression patterns of genes related to cold tolerance in Rosa hybrida

文献类型： 外文期刊

第一作者： Wang, Hongli

作者： Wang, Hongli;Cheng, Xi;Shi, Qiyu;Xu, Jie;Chen, Dongliang;Luo, Chang;Liu, Hua;Huang, Conglin;Shi, Qiyu;Cao, Li

作者机构：

关键词： Rosa L; cold tolerance; RNA-Seq; qRT-PCR; resource evaluation

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.6； 五年影响因子：6.8 ）

ISSN： 1664-462X

年卷期： 2023 年 14 卷

页码：

收录情况： SCI

摘要： Members of the Rosa genus have a high ornamental value, but their cultivation area is limited by their sensitivity to cold temperatures. The aim of this study was to evaluate the cold tolerance of a range of Rosa materials, and then determine which genes were related to cold tolerance. Nine Rosa materials were subjected to a cold treatment. To identify genes related to cold tolerance, R. hybrida was treated at -15 & DEG;C for 10 min, and leaves collected before and after this treatment were collected for RNA-Seq analyses. The transcript profiles of four DEGs (POD17, NDUFA9, PMA1, and b-Amy1) in R. hybrida were determined by qRT-PCR at 0 h, 1 h, 2 h, and 3 h at -15 & DEG;C. Nine Rosa materials were subjected to a cold treatment, and the most cold-tolerant materials were identified as those that showed the lowest levels of electrolyte leakage and the best recovery after 30 d of growth. The most cold-tolerant materials were Rosa hybrida, Rosa rugosa 'Pingyin 12', and Rosa rugosa. In total, 204 significantly differentially expressed genes (DEGs) were identified, of which 88 were significantly up-regulated and 116 were significantly down-regulated under cold conditions. Gene Ontology classification and Kyoto Encyclopedia of Genes and Genomes pathway analyses showed that the DEGs were enriched in 57 pathways, especially starch and sucrose metabolism, phenylpropane biosynthesis, MAPK signaling, fructose and mannose metabolism, and oxidative phosphorylation. By transcriptional analysis, PMA1, which was related to H+ ATPase activity, was continuously up-regulated, but the transcript levels of POD17, NDUFA9, and & beta;-Amy1 fluctuated during the freezing treatment. This research uncovered scarce cold-resistant materials and layed the foundation for further research on the cold tolerance mechanism of Rosa plants and the breeding of cold-tolerant varieties.

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