Genome editing of a rice CDP-DAG synthase confers multipathogen resistance

文献类型： 外文期刊

第一作者： Sha, Gan

作者： Sha, Gan;Sun, Peng;Kong, Xiaojing;Han, Xinyu;Sun, Qiping;Zhao, Juan;Li, Yun;Yang, Lei;Wang, Yin;Gong, Qiuwen;Zhou, Yaru;Zhou, Wenqing;Chen, Xiaoyang;Zheng, Lu;Zhang, Wanying;Qin, Ziting;Li, Guotian;Sha, Gan;Sun, Peng;Kong, Xiaojing;Han, Xinyu;Sun, Qiping;Zhao, Juan;Li, Yun;Yang, Lei;Wang, Yin;Gong, Qiuwen;Zhou, Yaru;Zhou, Wenqing;Chen, Xiaoyang;Zheng, Lu;Zhang, Wanying;Qin, Ziting;Li, Guotian;Sha, Gan;Sun, Peng;Kong, Xiaojing;Han, Xinyu;Sun, Qiping;Zhao, Juan;Li, Yun;Yang, Lei;Wang, Yin;Gong, Qiuwen;Zhou, Yaru;Zhou, Wenqing;Chen, Xiaoyang;Zheng, Lu;Zhang, Wanying;Qin, Ziting;Li, Guotian;Sha, Gan;Sun, Peng;Kong, Xiaojing;Han, Xinyu;Sun, Qiping;Zhao, Juan;Li, Yun;Yang, Lei;Wang, Yin;Gong, Qiuwen;Zhou, Yaru;Zhou, Wenqing;Chen, Xiaoyang;Zheng, Lu;Zhang, Wanying;Qin, Ziting;Li, Guotian;Fouillen, Laetitia;Boutte, Yohann;Jain, Rashmi;Ronald, Pamela C.;Li, Guotian;Jain, Rashmi;Ronald, Pamela C.;Li, Guotian;Jain, Rashmi;Mortimer, Jenny C.;Ronald, Pamela C.;Li, Guotian;Gao, Jie;Xie, Kabin;Guo, Liang;Li, Qiang;Huang, Renliang;Zhou, Qi;Chiu, Tsan-Yu;Zeng, Qingdong;Kang, Zhensheng;Xu, Jiandi;Mortimer, Jenny C.;Ronald, Pamela C.;Zhao, Juan;Chen, Xiaoyang

作者机构：

期刊名称：NATURE （ 影响因子：64.8； 五年影响因子：60.9 ）

ISSN： 0028-0836

年卷期： 2023 年 618 卷 7967 期

页码：

收录情况： SCI

摘要： The discovery and application of genome editing introduced a new era of plant breeding by giving researchers efficient tools for the precise engineering of crop genomes(1). Here we demonstrate the power of genome editing for engineering broad-spectrum disease resistance in rice (Oryza sativa). We first isolated a lesion mimic mutant (LMM) from a mutagenized rice population. We then demonstrated that a 29-base-pair deletion in a gene we named RESISTANCE TO BLAST1 (RBL1) caused broad-spectrum disease resistance and showed that this mutation caused an approximately 20-fold reduction in yield. RBL1 encodes a cytidine diphosphate diacylglycerol synthase that is required for phospholipid biosynthesis(2). Mutation of RBL1 results in reduced levels of phosphatidylinositol and its derivative phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P-2). In rice, PtdIns(4,5)P-2 is enriched in cellular structures that are specifically associated with effector secretion and fungal infection, suggesting that it has a role as a disease-susceptibility factor(3). By using targeted genome editing, we obtained an allele of RBL1, named RBL1(Delta 12), which confers broad-spectrum disease resistance but does not decrease yield in a model rice variety, as assessed in small-scale field trials. Our study has demonstrated the benefits of editing an LMM gene, a strategy relevant to diverse LMM genes and crops.

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