A phosphorylation-regulated NPF transporter determines salt tolerance by mediating chloride uptake in soybean plants

文献类型： 外文期刊

第一作者： Wu, Yunzhen

作者： Wu, Yunzhen;Yuan, Jingya;Shen, Like;Li, Zhuomeng;Cao, Hongwei;Zhu, Lin;Liu, Dan;Sun, Yalu;Zhang, Wenhua;Zhang, Qun;Wu, Yunzhen;Yuan, Jingya;Shen, Like;Li, Zhuomeng;Cao, Hongwei;Zhu, Lin;Liu, Dan;Sun, Yalu;Wang, Wubin;Zhang, Wenhua;Gai, Junyi;Zhang, Qun;Li, Qinxue;Jia, Qianru;Chen, Huatao;Kudla, Joerg

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关键词： Chloride Homeostasis; NPF Transporters; Phosphorylation; Soybean; Salt Tolerance

期刊名称：EMBO JOURNAL （ 影响因子：8.3； 五年影响因子：10.6 ）

ISSN： 0261-4189

年卷期： 2025 年 44 卷 3 期

页码：

收录情况： SCI

摘要： Chloride (Cl-) ions cause major damage to crops in saline soils. Understanding the key factors that influence Cl- uptake and translocation will aid the breeding of more salt-tolerant crops. Here, using genome-wide association study and transcriptomic analysis, we identified a NITRATE TRANSPORTER 1 (NRT1)/PEPTIDE TRANSPORTER family (NPF) protein, GmNPF7.5, as the dominant gene locus influencing Cl- homeostasis in soybean (Glycine max). A natural SNP variation resulted in two haplotypes (GmNPF7.5HapA and GmNPF7.5HapB), which was associated with Cl- content. GmNPF7.5HapA mediated Cl- or nitrate (NO3-) uptake in a pH-dependent manner and exhibited higher permeability for Cl- over NO3-. The suppression of GmNPF7.5HapA expression decreased Cl- accumulation and salt damage in plants, whereas its overexpression showed the opposite effects. The elite haplotype GmNPF7.5HapB diminished Cl- transport activity independently from NO3- permeability, thus enhancing soybean salt tolerance. Furthermore, the protein kinase GmPI4K gamma 4 could phosphorylate GmNPF7.5, which repressed Cl- uptake without affecting NO3- permeability. Our findings define a regulatory mechanism for Cl- control under NaCl stress, providing a strategy for the improvement of salt tolerance in soybean plants.

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