Integrative omics analysis elucidates the genetic basis underlying seed weight and oil content in soybean

文献类型： 外文期刊

第一作者： Yuan, Xiaobo

作者： Yuan, Xiaobo;Jiang, Xinyu;Zhang, Mengzhu;Wang, Longfei;Jiao, Wu;Mao, Junrong;Ye, Wenxue;Song, Qingxin;Chen, Huatao

作者机构：

期刊名称：PLANT CELL （ 影响因子：11.6； 五年影响因子：12.9 ）

ISSN： 1040-4651

年卷期： 2024 年 36 卷 6 期

页码：

收录情况： SCI

摘要： Synergistic optimization of key agronomic traits by traditional breeding has dramatically enhanced crop productivity in the past decades. However, the genetic basis underlying coordinated regulation of yield- and quality-related traits remains poorly understood. Here, we dissected the genetic architectures of seed weight and oil content by combining genome-wide association studies (GWAS) and transcriptome-wide association studies (TWAS) using 421 soybean (Glycine max) accessions. We identified 26 and 33 genetic loci significantly associated with seed weight and oil content by GWAS, respectively, and detected 5,276 expression quantitative trait loci (eQTLs) regulating expression of 3,347 genes based on population transcriptomes. Interestingly, a gene module (IC79), regulated by two eQTL hotspots, exhibited significant correlation with both seed weigh and oil content. Twenty-two candidate causal genes for seed traits were further prioritized by TWAS, including Regulator of Weight and Oil of Seed 1 (GmRWOS1), which encodes a sodium pump protein. GmRWOS1 was verified to pleiotropically regulate seed weight and oil content by gene knockout and overexpression. Notably, allelic variations of GmRWOS1 were strongly selected during domestication of soybean. This study uncovers the genetic basis and network underlying regulation of seed weight and oil content in soybean and provides a valuable resource for improving soybean yield and quality by molecular breeding. Integration of multiple association studies reveals genetic loci, coexpression modules, and candidate genes that are significantly associated with simultaneous regulation of seed weight and oil content.

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