Integrated Transcriptomic-Metabolomic Analysis Reveals the Effect of Different Light Intensities on Ovarian Development in Chickens

文献类型： 外文期刊

第一作者： Zhou, Xiaoli

作者： Zhou, Xiaoli;Xu, Yuhang;Fang, Cheng;Ye, Chutian;Liang, Weiming;Fan, Zhexia;Ma, Xuerong;Liu, Aijun;Zhang, Xiquan;Luo, Qingbin;Zhou, Xiaoli;Xu, Yuhang;Fang, Cheng;Ye, Chutian;Liang, Weiming;Fan, Zhexia;Ma, Xuerong;Liu, Aijun;Zhang, Xiquan;Luo, Qingbin

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关键词： light intensity; ovarian development; transcriptomics; metabolomics; partridge chicken

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：4.9； 五年影响因子：5.7 ）

ISSN： 1661-6596

年卷期： 2024 年 25 卷 16 期

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收录情况： SCI

摘要： Light is a key environmental factor regulating reproduction in avians. However, the mechanism of light intensity regulating ovarian development is still unclear. In this study, 5-week-old (5 wk) partridge broiler breeders were randomly divided into a low-light-intensity group (LL group) and a natural-light-intensity group (NL group) (n = 100). In the rearing period (5 wk to 22 wk), the light intensity of the LL group and NL group were 0.41 +/- 0.05 lux and 45.39 +/- 1.09 lux, and in the laying period (23 wk to 32 wk) they were 23.92 +/- 0.06 lux and 66.93 +/- 0.76 lux, respectively. Samples were collected on 22 wk and 32 wk. The results showed that the LL group had a later age at first egg and a longer laying period than the NL group. Serum P4 and LH levels in the LL group were higher than in the NL group on 22 wk (p < 0.05). On 32 wk, P4, E2, LH and FSH levels in the LL group were lower than in the NL group (p < 0.05). Ovarian transcriptomics and metabolomics identified 128 differentially expressed genes (DEGs) and 467 differential metabolites (DMs) on 22 wk; 155 DEGs and 531 DMs on 32 wk between two groups. An enrichment analysis of these DEGs and DMs identified key signaling pathways, including steroid hormone biosynthesis, neuroactive ligand-receptor interaction. In these pathways, genes such as CYP21A1, SSTR2, and NPY may regulate the synthesis of metabolites, including tryptamine, triglycerides, and phenylalanine. These genes and metabolites may play a dominant role in the light-intensity regulation of ovarian development and laying performance in broiler breeders.

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