Lysine 98 in NAC20/NAC26 transcription factors: a key regulator of starch and protein synthesis in rice endosperm

文献类型： 外文期刊

第一作者： Xu, Shanbin

作者： Xu, Shanbin;Zhang, Yu;Cai, Hongping;He, Yuzhe;Chen, Laibao;Zhang, Guiping;Chen, Rongbo;Gu, Chuanwei;Teng, Xuan;Duan, Erchao;Jiang, Ling;Wang, Yihua;Dong, Hui;Wan, Jianmin;Ren, Yulong;Wan, Jianmin;Teng, Xuan;Duan, Erchao;Jiang, Ling;Wang, Yihua;Dong, Hui;Wan, Jianmin

作者机构：

关键词： Rice; Starch; SSPs; Floury endosperm; NAC transcription factor

期刊名称：PLANT SCIENCE （ 影响因子：4.1； 五年影响因子：5.1 ）

ISSN： 0168-9452

年卷期： 2024 年 349 卷

页码：

收录情况： SCI

摘要： Starch and proteins are main storage product to determine the appearance, cooking, texture, and nutritional quality of rice (Oryza sativa L.). OsNAC20 and OsNAC26, as pivotal transcription factors, redundantly regulate the expression of genes responsible for starch and protein synthesis in the rice endosperm. Any knockout of OsNAC20 or OsNAC26 did not result in visible endosperm defects. In this study, we had isolated and characterized a mutant named as floury endosperm25 (flo25). The caryopsis of the flo25 mutant exhibits a floury endosperm, accompanied by reductions in both the 1000-grain weight and grain length, as well as diminished levels of total starch and protein. Through map-based cloning, it was determined that FLO25 encodes a NAM, ATAF, and CUC (NAC) transcription factors, namely OsNAC26, with a lysine to asparagine substitution at position 98 in the flo25 mutant. Remarkably, lysine 98 is conserved across plants species, and this mutation does not alter the subcellular localization of OsNAC26 but significantly attenuates its transcriptional activity and its ability to activate downstream target genes. Furthermore, the mutant protein encoded by OsNAC26(-flo25) could interact with OsNAC20, disrupting the native interaction between OsNAC20 proteins. Additionally, when lysine 98 is substituted with asparagine in OsNAC20, the resulting mutant protein, OsNAC20(K98N), similarly disrupts the interaction between OsNAC26 proteins. Collectively, these findings underscore the pivotal role of Lysine 98 (K) in modulating the transcriptional activity of NAC20/NAC26 within the rice endosperm.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序