The Cry2Aa protein is not enough to pose a threat to Pardosa astrigera

文献类型： 外文期刊

第一作者： Liu, Weijiao

作者： Liu, Weijiao;Yu, Qiqing;Nasir, Muhammad;Zhu, Xiangzhen;Wang, Li;Zhang, Kaixin;Li, Dongyang;Ji, Jichao;Luo, Junyu;Cui, Jinjie;Gao, Xueke;Liu, Weijiao;Yu, Qiqing;Nasir, Muhammad;Zhu, Xiangzhen;Iqbal, Muhmmad Shahid;Elumalai, Punniyakotti;Wang, Li;Zhang, Kaixin;Li, Dongyang;Ji, Jichao;Luo, Junyu;Cui, Jinjie;Gao, Xueke;Liu, Weijiao;Yu, Qiqing;Luo, Junyu;Cui, Jinjie;Gao, Xueke;Nasir, Muhammad;Iqbal, Muhmmad Shahid

作者机构：

关键词： Cry2Aa protein; 16S rRNA; Symbiotic bacteria; Fitness

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）

ISSN： 0141-8130

年卷期： 2025 年 301 卷

页码：

收录情况： SCI

摘要： The widespread commercialization of genetically modified (GM) crops makes it important to assess the potential impact of Bacillus thuringiensis (Bt) on non-target organisms. Pardosa astrigera is an important predator in agroforestry ecosystems, and female and male spiders may react differently to Bt toxins due to their different activity habits and nutritional requirements. In this study, we found that exposure to Cry2Aa protein did not affect the survival and body weight of P. astrigera during growth and development. However, according to 16S rRNA sequencing results of the P. astrigera adults, Cry2Aa protein not only changed the diversity of symbiont bacteria, but also changed its symbiont composition. During feeding on prey without Bt artificial feed, the dominant communities in female and male adults were Actinobacteria and Corynebacterium-1, respectively. Feeding on prey containing Cry2Aa protein, Firmicutes were the dominant phyla. At the genus level, Cry2Aa protein significantly increased the relative abundance of Enterococcus and became the dominant genus in females only. In addition, Bacillus, Weissella and other symbiotic bacteria had significant changes in females. In terms of species composition, sex differences resulted in the absence of different types of symbiotic bacteria. Functional analysis of enrichment pathways showed significant changes in various metabolic pathways such as "Carbohydrate metabolism" and "Nucleotide metabolism", and there are differences between the sexes. These findings provide new data information and support for revealing the different strategies of spiders to cope with Cry2Aa protein based on sex differences, and also provide new data information and support for environmental safety assessment of GM crops.

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