Antioxidants improve β-cypermethrin degradation by alleviating oxidative damage and increasing bioavailability by Bacillus cereus GW-01

文献类型： 外文期刊

第一作者： Xie, Yuxuan

作者： Xie, Yuxuan;Gong, Lanmin;Liu, Shan;Yan, Jisha;Zhao, Sijia;Liu, Gang;Zhao, Jiayuan;Xie, Yuxuan;Liu, Shan;Yan, Jisha;Zhao, Sijia;Liu, Gang;Zhao, Jiayuan;Xia, Chen;Li, Ke;Mazhar, Muhammad Waqar;Mazhar, Muhammad Waqar

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关键词： ss-cypermethrin; Cell surface hydrophobicity; Biofilm Fatty acid; Antioxidants

期刊名称：ENVIRONMENTAL RESEARCH （ 影响因子：8.3； 五年影响因子：8.2 ）

ISSN： 0013-9351

年卷期： 2023 年 236 卷

页码：

收录情况： SCI

摘要： Microbial degradation of pesticide residues has the potential to reduce their hazards to human and environmental health. However, in some cases, degradation can activate pesticides, making them more toxic to microbes. Here we report on the ss-cypermethrin (ss-CY) toxicity to Bacillus cereus GW-01, a recently described ss-CY degrader, and effects of antioxidants on ss-CY degradation. GW-01 exposed to ss-CY negatively affected the growth rate. The highest maximum specific growth rate (mu m) appeared at 25 mg/L ss-CY. ss-CY induced the oxidative stress in GW-01. The activities of superoxide dismutase (SOD), catalyse (CAT), and glutathione-S-transferase (GST) were significantly higher than that in control (p < 0.01); but they are decreased as growth phase pronged, which is contrary to the ss-CY degradation by GW-01 cells obtaining from various growth phase. Ascorbic acid (Vc), tea polyphenols (TP), and adenosine monophosphate (AMP) improved the degradation through changing the physiological property of GW-01. TP and AMP prompted the expression of gene encoding ss-CY degradation in GW-01, while Vc does the opposite. Biofilm formation was significantly inhibited by ss-CY, while was significantly enhanced by certain concentrations of TP and AMP (p < 0.05); while cell surface hydrophobicity (CSH) was negatively associated with ss-CY concentrations from 25 to 100 mg/L, and these 4 antioxidants all boosted the CSH. Cells grown with ss-CY had lower levels of saturated fatty acids but increased levels of some unsaturated and branched fatty acids, and these antioxidants alleviated the FA composition changes and gene expression related with FA metabolism. We also mined transcriptome analyses at lag, logarithmic, and stationary phases, and found that ss-CY induced oxidative stress. The objective of this study was to elaborate characteristics in relation to the microbial resistance of pesticide poisoning and the efficiency of pesticide degradation, and to provide a promising method for improving pesticide degradation by microbes.

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