Antiviral effect of recombinant equine interferon-gamma on several equine viruses

文献类型: 外文期刊

第一作者: Sentsui, Hiroshi

作者: Sentsui, Hiroshi;Wu, Donglai;Murakami, Kenji;Kondo, Takashi;Matsumura, Tomio

作者机构:

关键词: Baculovirus;Equine virus;Horse;Interferon-gamma;Recombinant interferon

期刊名称:VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY ( 影响因子:2.046; 五年影响因子:2.217 )

ISSN: 0165-2427

年卷期: 2010 年 135 卷 1-2 期

页码:

收录情况: SCI

摘要: Recombinant equine interferon-gamma (reIFN-gamma) was prepared using a baculovirus expression system and its antiviral activity was investigated using several equine viruses. The reIFN-gamma suppressed the replication of all equine viruses used in the present experiment in horse cell cultures, but did not affect the growth of host cells at concentrations of less than 1000 u/ml. A strong antiviral effect was observed, especially against RNA viruses. Equine picornavirus, equine rhinovirus and equine arteritis virus could not be propagated at all in 100 u/ml reIFN-gamma when 100 TCID(50) of infective viruses was inoculated to cultivated horse cells. DNA viruses, equine herpesvirus types 1, 2, 3 and 4 and equine adenovirus, were less sensitive to reIFN-gamma but their growth became less than 1/100 in the cells treated with 100 u/ml reIFN-gamma compared to untreated cells. The antiviral effects were decreased in the cells of heterologous species and more than 1000 u/ml reIFN-gamma was required to induce an antiviral effect. (C) 2009 Elsevier B.V. All rights reserved.

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