Development and optimization of a DNA-based reverse genetics systems for epizootic hemorrhagic disease virus

文献类型： 外文期刊

第一作者： Guo, Yunze

作者： Guo, Yunze;Xu, Qingyuan;Wu, Donglai;Bu, Zhigao;Sun, Encheng;Guo, Yunze;Pretorius, Jakobus M.;Theron, Jacques

作者机构：

期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.574； 五年影响因子：2.466 ）

ISSN： 0304-8608

年卷期： 2020 年 165 卷 5 期

页码：

收录情况： SCI

摘要： Epizootic hemorrhagic disease virus (EHDV) is a member of the genus Orbivirus, family Reoviridae, and has a genome consisting of 10 linear double-stranded (ds) RNA segments. The current reverse genetics system (RGS) for engineering the EHDV genome relies on the use of in vitro-synthesized capped viral RNA transcripts. To obtain more-efficient and simpler RGSs for EHDV, we developed an entirely DNA (plasmid or PCR amplicon)-based RGS for viral rescue. This RGS enabled the rescue of infectious EHDV from BSR-T7 cells following co-transfection with seven helper viral protein expression plasmids and 10 cDNA rescue plasmids or PCR amplicons representing the EHDV genome. Furthermore, we optimized the DNA-based systems and confirmed that some of the helper expression plasmids were not essential for the recovery of infectious EHDV. Thus, DNA-based RGSs may offer a more efficient method of recombinant virus recovery and accelerate the study of the biological characteristics of EHDV and the development of novel vaccines.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序