Detection of Newcastle disease virus using nucleic acid sequence-based amplification

文献类型: 外文期刊

第一作者: Cui, Shang-Jin

作者: Cui, Shang-Jin;Fung, Yin-Wan Wendy;Lau, Lok Ting;Liu, Wen-Bin;Wang, Yun-Feng;Tong, Guang-Zhi;Chen, Jianguo;Yu, Albert Cheung Hoi

作者机构:

关键词: Newcastle disease;NASBA;NDV;RNA amplicons;surveillance;disease screening

期刊名称:BIOLOGICALS ( 影响因子:1.856; 五年影响因子:2.05 )

ISSN: 1045-1056

年卷期: 2007 年 35 卷 1 期

页码:

收录情况: SCI

摘要: Newcastle disease (ND) is a contagious and widespread avian disease affecting most species of birds. ND virus (NDV) is the only member of the avian paramyxovirus serotype 1 (APMV1) causing ND outbreak in bird flocks. The technique of nucleic acid sequence-based amplification (NASBA) is a potential method to rapidly and reliably detect NDV isolates. Here, we describe an effective and unprecedented method for detecting NDV strains of all pathotypes. A conserved region of the fusion protein gene was used for designing oligonucleotides specific to all NDV pathotypes. The dynamic range of this NDV NASBA detection method is comparable to virus culture and therefore the NDV NASBA method is a potential alternative for NDV screening and surveillance. (c) 2005 The International Association for Biologicals. Published by Elsevier Ltd. All rights reserved.

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