Differential glycosylation of rhLf expressed in the mammary gland of transgenic mice

文献类型: 外文期刊

第一作者: Zhao, CJ

作者: Zhao, CJ;Liu, ZL;Fan, BL;Dai, YP;Wang, LL;Zheng, M;Wang, ML;Niu, HL;Xi, FG;Li, N;Zhang, DL

作者机构: China Agr Univ, Coll Biol Sci, State Key Labs Agrobiotechnol, Beijing 100094, Peoples R China;China Agr Univ, Coll Biol Sci, State Key Labs Agrobiotechnol, Beijing 100094, Peoples R China^China Agr Univ, Coll Anim Sci & Technol, Key Labs Minist Agr PR CHina Anim Breeding & Gene, Beijing 100094, Peoples R China^China Agr Sci Acad, Atom Energy Applicat Inst, Beijing, Peoples R China

关键词: human lactoferrin;recombinant human lactoferrin;glycosylation

期刊名称:ANIMAL BIOTECHNOLOGY ( 2020影响因子:2.282; 五年影响因子:1.702 )

ISSN: 1049-5398

年卷期: 2006 年 17 卷 1 期

页码:

收录情况: SCI

摘要: Differential glycosylation of natural hLf and rhLf from hLf-transgenic mice, which harbored a 146 Kb BAC insert that includes the intact hLf gene sequence, was studied in the present report. There were significant differences between the immunoblotting results of rhLf and natural hLf, which were denatured with nonreducing SDS sample buffer. The differences disappeared after rhLf and natural hLf samples were digested with N-glycosidase F, respectively. The results showed that there were significant differences ( P 0.01) between the glycosylation of natural hLf and rhLf that were purified, respectively, from milk samples of seven hLf-transgenic mouse lines.

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