Genetic analysis and mapping of rice (Oryza sativa L.) male-sterile (OsMS-L) mutant

文献类型： 外文期刊

第一作者： Liu, HS

作者： Liu, HS;Chu, HW;Li, H;Wang, HM;Wei, JL;Li, N;Ding, SY;Huang, H;Ma, H;Huang, CF;Luo, D;Yuang, Z;Liu, JH;Zhang, DB

作者机构：

关键词： rice (Oryza sativa L.);mutants;male-sterility;gene mapping;molecular marker

期刊名称：CHINESE SCIENCE BULLETIN （ 影响因子：1.649； 五年影响因子：1.738 ）

ISSN： 1001-6538

年卷期： 2005 年 50 卷 2 期

页码：

收录情况： SCI

摘要： A rice male-sterile mutant OsMS-L of japonica cultivar 9522 background, was obtained in M4 population treated with Co-60 gamma-Ray. Genetic analysis indicated that the male-sterile phenotype was controlled by a single recessive gene. Results of tissue section showed that at microspore stage, OsMS-L tapetum was retarded. Then tapetal cells expanded and microspores degenerated. No matured pollens were observed in OsMS-L anther locus. To map OsMS-L locus, an F-2 population was constructed from the cross between the OsMS-L (japonica) and LongTeFu B(indica). Firstly, the OsMS-L locus was roughly mapped between two SSR markers, RM109 and RM7562 on chromosome 2. And then eleven polymorphic markers were developed for further fine fine-mapping. At last the OsMS-L locus was mapped between the two InDel markers, Lhs10 and Lhs6 with genetic distance of 0.4 cM, respectively. The region was delimited to 133 kb. All these results were useful for further cloning and functional analysis of OsMS-L.

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