Oral immunization of mice with plant-derived fimbrial adhesin FaeG induces systemic and mucosal K88ad enterotoxigenic Escherichia coli-specific immune responses

文献类型： 外文期刊

第一作者： Liang, WQ

作者： Liang, WQ;Huang, YH;Yang, XH;Zhou, Z;Pan, AH;Qian, BJ;Huang, C;Chen, JX;Zhang, DB

作者机构：

关键词： enterotoxigenic Escherichia coli;proteinaceous fimbriae;recombinant FaeG;transgenic tobacco

期刊名称：FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY （ 影响因子：3.078； 五年影响因子：2.747 ）

ISSN： 0928-8244

年卷期： 2006 年 46 卷 3 期

页码：

收录情况： SCI

摘要： The importance of adhesins in pathogenicity has resulted in them being useful targets in the defense against bacterial infections. To produce edible vaccines against piglet diarrhea caused by enterotoxigenic Escherichia coli (ETEC), plants were genetically engineered to produce recombinant fimbrial adhesin FaeG. To evaluate the efficacy of the edible vaccine FaeG in mice, the soluble protein extracts were examined by about 15 mu g recombinant FaeG for each oral immunization dose per mouse. After four doses of vaccination, both IgG and IgA antibodies specific to K88ad fimbriae were elicited in serum, and specific IgA antibodies were also evoked in feces of the immunized mice. Moreover, visible K88ad ETEC agglutination by the specific serum from the immunized mice was observed, implying the antibody was highly specific and effective. Results from an in vitro villous-adhesion assay further confirmed that serum antibodies of the immunized mice could inhibit K88ad ETEC from adhering to pig intestinal receptors, further demonstrating the oral immune efficacy of the plant-derived FaeG. This study provides a promising, noninvasive method for vaccinating swine by feeding supplements of transgenic plant. Moreover, the low cost and ease of delivery of this edible ETEC vaccine will facilitate its application in economically disadvantaged regions.

分类号：

  • 相关文献

[1]Protective Immune Response of Bacterially-Derived Recombinant FaeG in Piglets. Huang Yahong,Liang, Wanqi,Pan, Aihu,Zhou, Zhiai,Wang, Qiang,Huang, Cheng,Chen, Jianxiu,Zhang, Dabing.

[2]Induction of specific immune responses in piglets by intramuscular immunization with fimbrial adhesin FaeG expressed in Lactococcus lactis. Liu, Shujie,Li, Yongming,Xu, Ziwei. 2013

[3]Subcutaneous or Oral Immunization of Mice with Lactococcus lactis Expressing F4 Fimbrial Adhesin FaeG. Liu, Shujie,Li, Yongming,Xu, Ziwei,Wang, Yicheng. 2013

[4]Isolation and Characterization of Lytic Phage vB_EcoM_JS09 against Clinically Isolated Antibiotic-Resistant Avian Pathogenic Escherichia coli and Enterotoxigenic Escherichia coli. Zhou, Yan,Bao, Hongduo,Zhang, Hui,Wang, Ran.

[5]CpG oligodeoxynucleotide protect neonatal piglets from challenge with the enterotoxigenic E. coli. Cai, Haiming,Shi, Juqing,Chu, Pingping,Huang, Chaoyuan,Yang, Jun,Zhang, Linghua,Kuang, Zheshi,Zhao, Xiangjie,Huang, Kuiying,Ming, Feiping,Xia, Fenggeng.

[6]In Vitro Evaluation of Swine-Derived Lactobacillus reuteri: Probiotic Properties and Effects on Intestinal Porcine Epithelial Cells Challenged with Enterotoxigenic Escherichia coli K88. Wang, Zhilin,Wang, Li,Chen, Zhuang,Ma, Xianyong,Yang, Xuefen,Zhang, Jian,Jiang, Zongyong.

[7]Overexpression of CaHSP26 in transgenic tobacco alleviates photoinhibition of PSII and PSI during chilling stress under low irradiance. Guo, Shang-Jing,Zhou, Hai-Yan,Zhang, Xian-Sheng,Li, Xin-Guo,Meng, Qing-Wei. 2007

[8]Overexpression of a tomato flavanone 3-hydroxylase-like protein gene improves chilling tolerance in tobacco. Zhang, Song,Wang, Guo-Dong,Kong, Fan-Ying,Meng, Chen,Deng, Yong-Sheng.

[9]Identification and differential expression of two isogenes encoding 1-deoxy-D-xylulose 5-phosphate reductoisomerase in Glycine max. Zhang, Man,Li, Kai,Liu, Jianyu,Yu, Deyue,Zhang, Man. 2012

[10]A Cu/Zn superoxide dismutase gene from Saussurea involucrata Kar. & Kir., SiCSD, enhances drought, cold, and oxidative stress in transgenic tobacco. Zhang, L.,Sun, L.,Zhang, L.,Qiu, H.,Liu, C.,Wang, A.,Zhu, J.,Deng, F.. 2017

[11]Combinational transformation of three wheat genes encoding fructan biosynthesis enzymes confers increased fructan content and tolerance to abiotic stresses in tobacco. Bie, Xiaomin,Wang, Ke,She, Maoyun,Du, Lipu,Zhang, Shuangxi,Gao, Xiang,Lin, Zhishan,Ye, Xingguo,Bie, Xiaomin,Zhang, Shuangxi,Li, Jiarui. 2012

[12]Expression of a wheat MYB gene in transgenic tobacco enhances resistance to Ralstonia solanacearum, and to drought and salt stresses. Liu, Hongxia,Zhou, Xianyao,Dong, Na,Liu, Xin,Zhang, Zengyan,Zhou, Xianyao,Zhang, Huaiyu.

[13]Transgenic tobacco expressing an Arisaema heterophyllum agglutinin gene displays enhanced resistance to aphids. Yao, JH,Zhao, XY,Qi, HX,Wan, BL,Chen, F,Sun, XF,Yu, SQ,Tang, KX. 2004

[14]Transcriptional Modulation of Ethylene Response Factor Protein JERF3 in the Oxidative Stress Response Enhances Tolerance of Tobacco Seedlings to Salt, Drought, and Freezing. Wu, Lijun,Zhang, Zhijin,Zhang, Haiwen,Huang, Rongfeng,Wu, Lijun,Wang, Xue-Chen,Wu, Lijun,Zhang, Zhijin,Zhang, Haiwen,Huang, Rongfeng,Wu, Lijun,Zhang, Zhijin,Zhang, Haiwen,Huang, Rongfeng.

[15]Evaluation of transgenic tobacco expressing two insecticidal genes to delay resistance development of Helicoverpa armigera. Fan, YL,Fan, XL,Shi, XP,Lu, MG.

[16]Overexpression of a peanut NAC gene, AhNAC4, confers enhanced drought tolerance in tobacco. Tang, G. Y.,Xu, P. L.,Shan, L.,Shao, F. X.,Liu, Z. J..

[17]Ectopic expression of peanut acyl carrier protein in tobacco alters fatty acid composition in the leaf and resistance to cold stress. Tang, G. -Y.,Liu, Z. -J.,Bi, Y. -P.,Shan, L.,Tang, G. -Y.,Liu, Z. -J.,Bi, Y. -P.,Shan, L.,Tang, G. -Y.,Liu, Z. -J.,Bi, Y. -P.,Shan, L.,Wei, L. -Q..

[18]Isolation and functional characterisation of CDPKs gene from Arachis hypogaea under salt stress. Li, Yan,Guo, Feng,Meng, Jing-Jing,Li, Xin-Guo,Li, Yan,Wan, Shu-Bo,Fang, Feng,Xia, Guang-Min.

[19]Peanut violaxanthin de-epoxidase alleviates the sensitivity of PSII photoinhibition to heat and high irradiance stress in transgenic tobacco. Yang, Sha,Meng, De-Yun,Hou, Lin-Lin,Li, Yan,Guo, Feng,Meng, Jing-Jing,Li, Xin-Guo,Wan, Shu-Bo,Meng, De-Yun,Hou, Lin-Lin.

[20]Overexpression of an MYB-Related Gene FvMYB1 from Fraxinus velutina Increases Tolerance to Salt Stress in Transgenic Tobacco. Li, Tian,Sun, Jingkuan,Bi, Yuping,Peng, Zhenying.

作者其他论文 更多>>

微信小程序